Self-assembled star-shaped chlorin-core poly(ε-caprolactone)-poly(ethylene glycol) diblock copolymer micelles for dual chemo-photodynamic therapies
Journal
Biomaterials
Journal Volume
29
Journal Issue
26
Pages
3599-3608
Date Issued
2008
Author(s)
Abstract
Amphiphilic 4-armed star-shaped chlorin-core diblock copolymers based on methoxy poly(ethylene glycol) (mPEG) and poly(ε-caprolactone) (PCL) were synthesized and characterized in this study. The synthesized photosensitizer-centered amphiphilic star block copolymer that forms assembled micelle-like structures can be used in a photodynamic therapy (PDT)-functionalized drug delivery system. Moreover, the hydrophobic chemotherapeutic agent, paclitaxel, can be trapped in the hydrophobic inner core of micelles. In our results, the star-polymer-formed micelle exhibited efficient singlet oxygen generation, whereas the hydrophobic photosensitizer failed due to aggregation in aqueous solution. The chlorin-core micelle without paclitaxel loading exhibited obvious phototoxicity in MCF-7 breast cancer cells with 7 J/cm2 or 14 J/cm2 light irradiation at a chlorin concentration of 125 μg/ml. After paclitaxel loading, the size of micelle increased from 71.4 nm to 103.2 nm. Surprisingly, these micelles were found to improve the cytotoxicity of paclitaxel significantly in MCF-7 cells after irradiation through a synergistic effect evaluated by median effect analysis. This functionalized micellar delivery system is a potential dual carrier for the synergistic combination of photodynamic therapy and chemotherapy for the treatment of cancer. Crown Copyright ? 2008.
Subjects
Cancer therapy; Micelle; Paclitaxel; Photodynamic therapy; Star-shaped copolymer
SDGs
Other Subjects
Block copolymers; Cells; Chemotherapy; Computer networks; Copolymerization; Drug delivery; Drug dosage; Drug therapy; Ethylene; Ethylene glycol; Gas generators; Glycols; Hydrophobicity; Micelles; Motion Picture Experts Group standards; Optical materials; Oxygen; Photodynamic therapy; Photosensitizers; Plasmons; Plastic products; Polyethylene glycols; Polymers; Solutions; (ethylene vinyl alcohol) copolymers; Amphiphilic(AP); Aqueous solutions; caprolactone (PCL); Chemotherapeutic agents; Chlorin; diblock; Diblock copolymer micelles; Drug delivery system (DDS); functionalized; Inner core; MCF-7 breast cancer cells; Methoxy poly(ethylene glycol); Micelle-like structures; Paclitaxel (PTX); Photo toxicity; Photodynamic therapy (PDT); Photosensitizer (PS); Polyethylene glycol (PEG); Self-assembled; Singlet oxygen generation (SOG); Star block copolymers; star polymers; Colloids; chlorin core poly(epsilon caprolactone)poly(ethylene glycol); copolymer; paclitaxel; singlet oxygen; aqueous solution; article; breast adenocarcinoma; cancer cell; cancer chemotherapy; cell viability; cytotoxicity; drug delivery system; drug potentiation; enzyme synthesis; female; human; human cell; hydrophobicity; light irradiance; micelle; photodynamic therapy; phototoxicity; priority journal; Biocompatible Materials; Cell Line, Tumor; Drug Carriers; Drug Therapy; Ethylene Glycols; Humans; Micelles; Microtubules; Molecular Structure; Paclitaxel; Photochemotherapy; Polyesters; Polymers; Porphyrins; Singlet Oxygen; Tubulin Modulators
Type
journal article