Application of Ling Zhi-8 (rLZ-8) and polysaccharide (PS-G) from Ganoderma lucidum as immunomodulators for alleviation of airway inflammation in mite-induced murine model of asthma
Date Issued
2007
Date
2007
Author(s)
Chang, Chih-Ling
DOI
en-US
Abstract
Ganoderma Lucidum, a China herb, has been widely used in China and other Asian countries. G. Lucidum has been reported to be effective in modulating immune functions. Ling Zhi-8 (LZ-8) is a protein derived from the fungus Ganoderma lucidum and the polysaccharide from Ganoderma Lucidum (PS-G) is a branched (1 → 6)-β-D-glucan moiety. Asthma is one of the most prevalent chronic airway inflammatory diseases in the world. The house dust mite, Dermatophagoides pteronyssinus (Der p), is the major allergen, and is the dominant species collected in Taiwan. Therefore, we like to investigate whether rLZ-8 and PS-G might exert a regulatory role in the treatment of allergic diseases. In this study, we first investigated the immunomodulatory effects of rLZ-8 and PS-G on BMDCs. The result showed that rLZ-8 and PS-G enhanced the expression of cell surface markers including CD86, CD83, and MHC Ⅱ, as well as the enhanced production of IL-12 p40 and IL-10. In addition, rLZ-8 or PS-G-treated BMDCs could promote T cell proliferation capacity, and increased T cell secretion of IFN-y. In animal Experiment Ⅰ, immunized female BALB/c mice were consistently intranasally received rLZ-8 and PS-G for treatment. In Experiment Ⅱ, mice were immunized (i.p.) injection of rDer p2 admixed with rLZ-8 or PS-G for investigating the preventive effect. Further, airway hyperresponsiveness, pulmonary function, TH1/TH2 cytokine production, and immunoglobulin levels will be followed. In conclusion, we demonstrated that rLZ-8 and PS-G effectively promoted the activation and maturation of immature BMDCs, suggesting that rLZ-8 and PS-G may possess a potential effect in regulating immune responses. In vivo result showed that both rLZ-8 and PS-G reduced airway hyperresponsiveness and serum IgE level. Moreover, PS-G could decrease the level of IL-4, IL-5 in BALF and splenocyte. rLZ-8 reduced IL-5 level in BALF. Although rLZ-8 and PS-G could modulate the response of asthma, the effect of these two molecules should be clarified in the future.
Subjects
氣喘
靈芝
多醣體
LZ-8
asthma
polysaccharides
Ganoderma lucidum
Type
other
File(s)![Thumbnail Image]()
Loading...
Name
ntu-96-R94450007-1.pdf
Size
23.31 KB
Format
Adobe PDF
Checksum
(MD5):f35db70b4bf7a2830857396ad0a36630