CLC-3氯離子通道在老鼠陰莖海綿體平滑肌之分佈與分子生物表現
Date Issued
2004
Date
2004
Author(s)
謝汝敦
DOI
922314B002333
Abstract
Chloride ion currents play a critical role in vascular smooth muscle electrophysiology.
Agonist-induced, inward chloride currents have now been characterized in numerous tissues,
including vessels. Recent studies demonstrate that interfering with either the distribution of
chloride across the membrane or the ability of chloride channels to open markedly suppresses
contractile responses of vascular smooth muscle to norepinephrine. Penile corpora own the
unique ability to inflate and deflate itself. Based on the obvious similarities in the structural and
pharmacological properties between cavernous myocytes and vascular smooth muscle cells, Clmight
be expected to also play a role in the regulation of the cavernous smooth muscle tone, and
thus, the erectile function. Our previous studies showed that the CLC-3 chloride channel proteins
could be detected in rat corpus cavernous smooth muscle tissue and cells via western blot. This
result means more extensive molecular studies of chloride channels in erectile tissue may be
necessary to distinguishing its possible physiological role. We therefore conducted a strategy
involving:
1. Immunohistochemistry
Detection of the distribution of CLC-3 chloride channel protein in rat corporal tissues and
culture cells.
2. Cloning and preparation of probes
The probes are synthesized by the primers by PCR amplification.
3.. Northern blot analysis
Detection of CLC-3 chloride channel gene expression in rat corporal tissues and culture cells.
The results revealed that the method of immunohistochemistry stain could identify the
distribution of CLC-3 chloride channel protein in could be detected on rat corporal tissues. The
probes could be synthesized by the primers by PCR amplification.
Subjects
ClC-3
Chloride channel
Corpus cavernous smooth muscle
Publisher
臺北市：國立臺灣大學醫學院泌尿科
Type
other
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