Expression of inhibitory natural killer receptors on tumor-infiltrating CD8+ T lymphocyte lineage in human endometrial carcinoma
Journal
International Journal of Gynecological Cancer
Journal Volume
15
Journal Issue
6
Pages
1073-1080
Date Issued
2005
Author(s)
Huang S.-C.
Hsu W.-C.
Chiou S.-H.
Abstract
To investigate the expression of natural killer receptors (NKRs) within the human tumor milieu, we directly examined the in vivo expressions of various NKRs on tumor-infiltrating lymphocytes (TILs) derived from human endometrial carcinoma (EC). In total, 22 patients with stage IA-IIIA EC were enrolled. TILs were isolated from tissue specimens by means of a mechanical dispersal technique. The subpopulations of immunocytes were quantified, and expressions of NKRs on CD8+ T cells were analyzed by triple-color flow cytometry. CD8+ T cells express higher ratios of CD94 and NKG2A in TILs than in peripheral blood mononuclear cells (PBMCs) in human EC. Flow cytometry reveals that 15.90% of CD3+CD8+ TILs compared with 2.10% of CD3+CD8+ PBMCs express the NKG2A molecules (P < 0.001). The percentage expressions of CD94 are 8.40% in CD3+CD8+ TILs and 3.80% in CD3+CD8+ PBMCs (P = 0.013). The numbers of CD8+ T cells expressing CD158b and NKB1 are higher in CD3 +CD8+ PBMCs in EC than in normal (CD158b: 10.70% vs 2.60%, P < 0.001; NKB1: 2.20% vs 0.40%, P = 0.018, respectively). Increased expression of CD94/NKG2A restricted to tumor-infiltrating CD8+ T cell subsets may shape the cytotoxic responses, which indicate a possible role of tumor escape from host immunity in human EC. ? 2005 IGCS.
SDGs
Other Subjects
CD3 antigen; CD8 antigen; CD94 antigen; natural killer cell receptor; natural killer cell receptor NKG2A; article; cancer staging; CD8+ T lymphocyte; cell differentiation; cell lineage; cell subpopulation; clinical article; controlled study; endometrium carcinoma; female; flow cytometry; human; human cell; human tissue; in vivo study; lymphocyte; peripheral blood mononuclear cell; priority journal; protein expression; tumor associated leukocyte; CD8-Positive T-Lymphocytes; Endometrial Neoplasms; Female; Humans; Killer Cells, Natural; Lymphocytes, Tumor-Infiltrating; Prospective Studies; Receptors, Immunologic; Tumor Escape
Type
journal article