由肝細胞癌差異表現基因之定量分析來進一步了解肝癌致病機轉
Other Title
Insight into hepatocellular carcinogenesis by quantitative analysis
of differential expressed genes in hepatocellular carcinoma
of differential expressed genes in hepatocellular carcinoma
Date Issued
2003
Date
2003
Author(s)
許金川
DOI
912315B002009
Abstract
Hepatocellular carcinoma (HCC) is one of the most common cancer in the world
and is the leading cause of cancer death in Taiwan. Chronic hepatitis B and recently the
hepatitis C viral infection are thought related to the development of HCC. However, the
basic molecular mechanism remained to be clarified.
In 2001, we have analyzed the transcriptional profiles of six HCC specimens using
Incyte Human GEM1 cDNA microarray which consists of ~9700 genes. Comparison of
expression profiles between HCC tumors and the corresponding nontumor tissues, we
identified 73 genes were up-regulated and 58 genes were down-regulated in tumor tissues.
The highly concordant overexpressed profile included transcripts involved in the function
of respiration control, protein synthesis, degradation, cytoskeleton and carcinogenesis.
Down-regulation of genes related to drug metabolism, differentiation and immune
response was observed in the majority of the tumors examined.
After comparing the LOH, CGH, and microarray data, we pick 3 differential
expression genes for further analysis. First, we extracted 44 pair of HCC RNA from
tumor part and corresponding nontumor part. RNA reverse transcribed to cDNA. In order
to validate cDNA microarray data, real-time quantitative polymerase chain reaction
(QPCR) was used to evaluate 3 gene (11 β-hydroxysteroid dehydrogenase, histidine-rich
glycoprotein and chaperonin CCT-γ) expressions. Highly correlation was observed
between these two methods. We also screen these 3 gene expression levels in a panel of
44 HCC specimens. 11 β-HSD and HRG were down-regulated in 55% (25/44), and 59%
(26/44) HCC cases respectively. CCT-γwas up-regulated in 59% (26/44) HCC cases.
The expression profiles of the 6 HCC cases provide valuable information for elucidation
of hepatocarcinogenesis, and also represent the potential biomarkers implicated in this
malignancy.
Subjects
hepatocellular carcinoma (HCC)
real-time quantitative polymerase chain
reaction (QPCR)
reaction (QPCR)
SDGs
Publisher
臺北市：國立臺灣大學醫學院內科
Type
report
File(s)![Thumbnail Image]()
Loading...
Name
912315B002009.pdf
Size
52.15 KB
Format
Adobe PDF
Checksum
(MD5):ae23c539dfd43dc5b5e1cd0512cafb61