Probing Lectin and Sperm with Carbohydrate-Modified Quantum Dots
Resource
ChemBioChem 6 (10): 1899-1905
Journal
ChemBioChem
Journal Issue
10
Pages
1899-1905
Date Issued
2005
Date
2005
Author(s)
Abstract
We report the encapsulation of quantum dots with biologically important β-N-acetylglucosamine (GlcNAc) in different ratios, together with studies of their specific/sensitive multivalent interactions with lectins and sperm by fluorimetry, transmission electron microscopy, dynamic light scattering microscopy, confocal imaging techniques, and flow cytometry. These GlcNAc-encapsulated quantum dots (QDGLNs) specifically bind to wheat germ agglutinin, and cause fluorescence quenching and aggregation. Further studies of QDGLNs and the mannose-encapsulated QDs (QDMANs) with sperm revealed site-specific interactions, in which QDGLNs bind to the head of the sperm, while QDMANs spread over the whole sperm body. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.
SDGs
Type
journal article