Silencing of p29 Affects DNA Damage Responses with UV Irradiation
Resource
Cancer Research 66 (17): 8484-8491
Journal
Cancer Research
Journal Volume
66
Journal Issue
17
Pages
8484-8491
Date Issued
2006
Date
2006
Author(s)
Abstract
Human p29 is a newly identified nuclear protein whose function is largely undetermined. We found that p29 associated with chromatin, interacted with MCM3, and localized with proliferating cell nuclear antigen foci in the S phase. Silencing of p29 using small interfering RNA duplexes reduced DNA synthesis and increased the expression of p107, a member of the RB family, and of cyclin-dependent kinase inhibitor p21, accompanied with a decreased expression of DNA polymerase α. Lethal events consisting of premature chromatin condensation with a reduced Chk1 phosphorylation were observed in p29-depleted cells in response to UV irradiation. Intriguingly, the phosphorylation of ataxia telangectasia-mutated kinases at S1981 was suppressed in p29-depleted HeLa cells with UV irradiation, but not in hydroxyurea- and ionizing radiation-treated cells. Taken together, these results reveal a novel function of p29 in the regulation of DNA replication checkpoint responses. ?2006 American Association for Cancer Research.
SDGs
Other Subjects
ATM protein; carrier protein; checkpoint kinase 1; cyclin dependent kinase inhibitor; cyclin dependent kinase inhibitor 1A; cycline; DNA directed DNA polymerase alpha; hydroxyurea; minichromosome maintenance protein 3; nuclear protein; protein p107; protein p29; retinoblastoma protein; small interfering RNA; unclassified drug; apoptosis; article; cell cycle S phase; chromatin; chromatin condensation; controlled study; depletion; DNA damage; DNA replication; DNA synthesis; gene silencing; genotoxicity; HeLa cell; human; human cell; ionizing radiation; nucleotide sequence; priority journal; protein expression; protein function; protein localization; protein phosphorylation; protein protein interaction; ultraviolet irradiation; Apoptosis; DNA; DNA Damage; DNA Replication; Gene Silencing; Hela Cells; Humans; Mitosis; Nuclear Proteins; RNA, Small Interfering; Ultraviolet Rays
Type
journal article
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