Separation rate and characteristics of extended-spectrum β-lactamase-producing bacterial isolates from dog and cat in National Taiwan University Veterinary Hospital during December 2011 to November 2013
Date Issued
2014
Date
2014
Author(s)
Chang, Chia-Wei
Abstract
Since the early 1980s, extended-spectrum β-lactamases (ESBLs) had become an emerging issue for antibiotic resistance. Production of ESBL confers resistance to the commonly used beta-lactam antimicrobials, but are inhibited by β-lactamases inhibitors. Escherichia coli and Klebsiella pneumoniae are the two major ESBL-producing bacteria. Several studies have revealed that the ESBL prevalence around the world is increasing in recent years, and the transmission route is changed. On the contrary, the isolation rates of resistance to ESBL among microorganisms from companion animals remained limited in Taiwan.
The objective of the present study was to examine and characterize the occurrence of ESBL-producing microorganisms in clinical samples of dogs/cats attending to National Taiwan University Veterinary Hospital (NTUVH) during December 2011 to November 2013. Combination disc tests using cefotaxime and ceftazidime (30 μg) disks, with and without clavulanic acid (10 μg) according to CLSI guidelines resulted in 60 isolates demonstrating an ESBL phenotype from 465 isolates included in this study. Twenty-six Escherichia coli, 16 Klebsiella pneumoniae, 10 Enterobacter spp., 3 Acinetobacter spp., 3 Serratia spp, and 2 Pseudomonas aeruginosa isolates exhibited ESBL phenotype. The ESBL prevalence was 12.9%. TEM and CTX-M were the two major ESBL genes, while CTX-M-1 was the most commonly found subgroup in CTX-M subtyping. Multilocus sequence typing (MLST) revealed that ST457 was the predominant sequence type in ESBL-producing E. coli.
Our result showed that the isolation rate of ESBL in NTUVH is comparatively higher than that from western countries. Increase of drug resistance may indicate the treatment difficulty in clinical practice. Gene typing and MLST results both showed the difference between human and companion animals in ESBL. Our results only represent the epidemiology of ESBL-producing bacteria from the companion animals in NTUVH, not the whole Taiwan’s companion animals.
Subjects
抗生素抗藥性
腸內菌科
超廣效性乙內醯胺酶
多基因位點序列分析
頭孢菌素
Type
thesis
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