Transplantation of human skeletal muscle-derived progenitor cells ameliorates knee osteoarthritis in streptozotocin-induced diabetic mice
Journal
Journal of Orthopaedic Research
Journal Volume
35
Journal Issue
9
Pages
1886-1893
Date Issued
2017
Author(s)
Abstract
The epidemiological and experimental evidence suggests that diabetes can be an independent risk factor for osteoarthritis. The osteoarthritis-like cartilage damage has been shown in streptozotocin-induced diabetic mice. The therapeutic effects of human skeletal muscle-derived progenitor cells (HSMPCs) on diabetic osteoarthritis still remain unclear. Here, we investigated the therapeutic potential of HSMPCs on diabetic knee osteoarthritis. The in vitro chondrogenic ability of HSMPCs was determined by pellet culture assay. Male mice were used to develop the model of streptozotocin-induced type 1 diabetes and its related osteoarthritis. HSMPCs were injected intra-articularly to rescue osteoarthritis. Protein expressions of advanced glycation end-products, cyclooxygenase-2, and type-2 collagen in tissues were determined by immunohistochemistry. The pellet culture assay showed that HSMPCs cultured in differentiation medium for chondrogenesis significantly produced larger pellets with an overproduction of extracellular matrix than in growth medium. In in vivo experiments, intra-articular injection of HSMPCs for 4 weeks significantly prevented the progression of degenerative changes in the cartilage of streptozotocin-induced diabetic mice, including an obvious increase of total articular cartilage thickness and a decrease of fibrous cartilage thickness. HSMPCs transplantation also exerted the decline in advanced glycation end-products and cyclooxygenase-2 protein expression, but increased the type-2 collagen protein expression in streptozotocin-induced osteoarthritic cartilages. Moreover, HSMPCs transplantation also inhibited the increased serum interleukin-6 and matrix metalloproteinase-3 levels in diabetic mice. These results demonstrated for the first time that HSMPCs transplantation ameliorates cartilage degeneration in diabetes-related osteoarthritis mice. These findings suggest that HSMPCs transplantation may apply as a potential therapeutic use of diabetes-related osteoarthritis. ? 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:1886–1893, 2017. ? 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.
SDGs
Other Subjects
advanced glycation end product; collagen type 2; cyclooxygenase 2; interleukin 6; stromelysin; adult; aged; animal experiment; animal model; Article; cartilage degeneration; cell differentiation; chondrogenesis; controlled study; extracellular matrix; female; fibrocartilage; human; human cell; human tissue; immunohistochemistry; in vitro study; in vivo study; insulin dependent diabetes mellitus; knee osteoarthritis; male; middle aged; mouse; muscle stem cell; nonhuman; priority journal; protein expression; risk factor; skeletal muscle cell; stem cell transplantation; streptozotocin-induced diabetes mellitus; therapy effect; animal; Arthritis, Experimental; complication; cytology; evaluation study; experimental diabetes mellitus; Institute for Cancer Research mouse; Osteoarthritis, Knee; skeletal muscle; xenograft; Animals; Arthritis, Experimental; Chondrogenesis; Diabetes Mellitus, Experimental; Diabetes Mellitus, Type 1; Humans; Male; Mice, Inbred ICR; Muscle, Skeletal; Osteoarthritis, Knee; Stem Cell Transplantation; Transplantation, Heterologous
Publisher
John Wiley and Sons Inc.
Type
journal article