Multiharmonic-generation biopsy of skin
Journal
Optics Letters
Journal Volume
28
Journal Issue
24
Pages
2488-2490
Date Issued
2003
Author(s)
Abstract
Because it avoids the in-focus photodamage and phototoxicity problem of two-photon-fluorescence excitation, multiharmonic-generation biopsy based on a 1200-1300-nm light source could provide a truly noninvasive and highly penetrative optical sectioning of skin. We study multiharmonic-generation biopsy of fixed mouse skin. Our preliminary study suggests that this technique could provide submicrometer-resolution deep-tissue noninvasive biopsy images in skin without the use of fluorescence and exogenous markers.
Other Subjects
Biopsy; Fluorescence; Harmonic generation; Laser damage; Light absorption; Light scattering; Medical imaging; Noninvasive medical procedures; Photons; Skin; Solid state lasers; Telescopes; Multiharmonic generation biopsy; Optical sectioning; Photodamage; Phototoxicity; Two photon fluorescence excitation; Two photon imaging; Laser tissue interaction
Type
journal article