Combined proteomic and metabolomic analyses of cerebrospinal fluid from mice with ischemic stroke reveals the effects of a Buyang Huanwu decoction in neurodegenerative disease
Journal
PLoS ONE
Journal Volume
14
Journal Issue
1
Pages
e0209184
Date Issued
2019
Author(s)
Abstract
Ischemic stroke is one of the most common causes of death worldwide and is a major cause of acquired disability in adults. However, there is still a need for an effective drug for its treatment. Buyang Huanwu decoction (BHD), a traditional Chinese medicine (TCM) prescription, has long been used clinically to aid neurological recovery after stroke. To establish potential clinical indicators of BHD efficacy in stroke treatment and prognosis, we conducted a combined proteomic and metabolomic analysis of cerebrospinal fluid (CSF) samples in a mouse stroke model. CSF samples were obtained from male mice with acute ischemic stroke induced by middle cerebral ischemic/reperfusion (CI/R) injury, some of which were then treated with BHD. Label-free quantitative proteomics was conducted using nano-LC-MS/ MS on an LTQ Orbitrap mass and metabolomic analysis was performed using nanoprobe NMR and UHPLC-QTOF-MS. The results showed that several proteins and metabolites were present at significantly different concentrations in the CSF samples from mice with CI/R alone and those treated with BHD. These belonged to pathways related to energy demand, inflammatory signaling, cytoskeletal regulation, Wnt signaling, and neuroprotection against neurodegenerative diseases. In conclusion, our in silico data suggest that BHD treatment is not only protective but can also ameliorate defects in pathways affected by neurological disorders. These data shed light on the mechanism whereby BHD may be effective in the treatment and prevention of stroke-related neurodegenerative disease. ? 2019 Hsu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
SDGs
Other Subjects
Buyang Huanwu decoction; neuroprotective agent; unclassified drug; buyang huanwu; herbaceous agent; animal experiment; animal model; animal tissue; Article; brain ischemia; cerebrospinal fluid analysis; controlled study; cytoskeleton; degenerative disease; drug effect; drug efficacy; drug response; liquid chromatography-mass spectrometry; male; metabolite; metabolomics; mouse; neuroprotection; nonhuman; nuclear magnetic resonance; proteomics; reperfusion injury; time of flight mass spectrometry; treatment outcome; ultra performance liquid chromatography; Western blotting; Wnt signaling; animal; brain ischemia; cerebrospinal fluid; cerebrovascular accident; Chinese medicine; disease model; Institute for Cancer Research mouse; metabolism; metabolomics; phytotherapy; protein cerebrospinal fluid level; proteomics; reperfusion injury; Animals; Brain Ischemia; Cerebrospinal Fluid Proteins; Disease Models, Animal; Drugs, Chinese Herbal; Male; Medicine, Chinese Traditional; Metabolic Networks and Pathways; Metabolomics; Mice; Mice, Inbred ICR; Phytotherapy; Proteomics; Reperfusion Injury; Stroke
Type
journal article