Feasibility and optimizing assessments on biogas and biomethane productions from E. coli fermenter effluent
Journal
Biomass and Bioenergy
Journal Volume
173
ISSN
0961-9534
Date Issued
2023-06
Author(s)
Ponnambalam Sabarikirishwaran
Ming-Yan Shen
Rameshprabu Ramaraj
Yuwalee Unpaprom
Chen-Yeon Chu
DOI
10.1016/j.biombioe.2023.106783
Abstract
his study pioneered the production of biomethane from E. coli fermenter effluent through anaerobic fermentation using wastewater treatment sludge with the aim of enhancing production by optimizing various factors affecting the anaerobic digestion process, including the Food-to-microorganism (F⋅M-1) ratio, pH level, and substrate Chemical Oxygen Demand (COD). Peak biomethane yield of 156 ± 2.63 ml⋅g CODadded-1 and volatile solids (VS) reduction up-to 89% was observed for the optimum condition of F∙M-1 ratio 2.6, pH 6.5 and COD 50 g⋅l-1. Results indicates adjusting the pH to 6.5 (for F⋅M-1 2.6) enhanced the inherent methanogenic homeostasis which counteracted the inhibitory effects of ammonia. This led to 22% increase in biomethane production compared to corresponding control sample. 16S rRNA analysis was conducted to study the diversity in the microbial communities of the samples. Kinetic study also employed modified Gompertz equation to model the cumulative biomethane production (R2 > 0.946).
Subjects
Ammonia inhibition
Biomethane
E. coli effluent
F/M ratio
Publisher
Elsevier BV
Description
Article number 106783
Type
journal article