H2O2 production and MnSOD expression in highly invasive potential A431 sub-line:Role in cell migration and invasion
Date Issued
2006
Date
2006
Author(s)
Hung, Hao-Hsiang
DOI
zh-TW
Abstract
Tumor metastasis has always been the main factor that causes the death of cancer patients, thus it is important to realize the mechanism of cancer metastasis. It has been appreciated for a number of years that H2O2 production is increased in malignant cancer cells. During tumor progression, H2O2 can activate MMP activity, cell-ECM adhesion, and subsequently promotes the capability of cell migration and cell invasion. The signaling pathway involved in these processes are thought to be achieved through redox modification of signaling molecules such as protein kinases and transcription factors. In the previous study from our laboratory, we have selected highly invasive tumor cell sub-line A431III from A431 by using Boyden Chamber Assay (unpublished data). In this study, we take the advantage of this highly invasive sub-line to further explore the role of H2O2 in cancer cells. We find that H2O2 production is increased in A431III sub-line compared to A431P. This result may attribute to differential expression of antioxidants, which were important in balancing cellular H2O2 levels. We find that MnSOD expression is increased whereas catalase is decreased in A431III sub-line compared to A431P. To further investigate the role of H2O2, we used MnSOD siRNA to suppress the expression of MnSOD. Interestingly, the amounts of H2O2 production were increased after transfection of MnSOD siRNA. The elevated H2O2 lead to increase the capabilities of cell migration, cell invasion, cell-ECM adhesion, and MMP expression. These results described above suggesting that H2O2 may act as an important messenger in regulating cell metastasis in A431 III sub-line.
Subjects
過氧化氫
MnSOD
H2O2
SDGs
Type
other
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