Survey on canine ehrlichiosis in Taipei by nested PCR
Date Issued
2004
Date
2004
Author(s)
Chang, Tzu-Chung
DOI
zh-TW
Abstract
Canine ehrlichiosis is a tick-borne disease caused by obligate intracellular parasites of the genus Ehrlichia. The diagnosis is difficult, because the clinical signs of canine ehrlichiosis are always not specific. Microscope and commercial kit are usually used for clinical diagnosis. However, some disadvantages exist in these diagnostic methods. We established nested-PCR with primers derived from the 16S rRNA gene sequence to study the infection rate, pathology, and coinfection with Babesia spp. of suspicious sample from animal hospitals in Taipei and Taipei Municipal Animal Shelter. We had designed five Ehrlichia-species-specific primers, and were able to amplify E. canis (29/174)and E. platys(2/174) of owned dogs as well as E. canis (4/31), E. platys(11/31)and E. equi (2/31) of stray dogs sampled from Taipei Municipal Animal Shelter. At the same time, we have sequenced the full 16S rRNA gene sequences of E. canis and E. platys, and compared with the other strains in gene bank by DNASTAR.
Subjects
艾利希氏體
Ehrlichiosis
Ehrlichia
Type
thesis
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