In vivo Photoacoustic Imaging with Multiple Selective Targeting Using Bioconjugated Gold Nanorods
Journal
SPIE International Symposium on Biomedical Optics
Date Issued
2008-01
Author(s)
Abstract
In this study, photoacoustic imaging is utilized to probe information from oncogene surface molecules of cancer cell with the aid of specific targeting. The ultimate goal is to provide prediction of clinical outcome and treatment response of anti-cancer drugs. Different from single targeting in most research, we accomplished multiple targeting to obtain a molecular profile potentially representing tumor characteristics or to locate the heterogeneous population in one lesion. By conjugating different antibodies to gold nanorods corresponding to different peak absorption bands, multiple targeting and simultaneous detection with photoacoustic imaging can be achieved with laser irradiation at the respective peak optical absorption wavelength. Her2 and EGFR were chosen as our primary target molecules. The targeting complex was evaluated in two types of oral cancer cells, OECM1 and Cal27. The OECM1 cell line overexpresses Her2 but has low expression of EGFR, while Cal27 cell line expresses both antibodies. Also, the targeting efficacy to OECM1 can be further improved by using mixed nanoprobes. The cancer cells were induced on the back of the mice by subcutaneous injection. The captured images show that both cancer cells exhibit a higher photoacoustic response (maximum 3 dB) than control groups with specific targeting, thus demonstrating the feasibility of multiple selective targeting with bioconjugated gold nanorods. Images of multiple targeting with mixed nanoprobes of OECM1 cells also reveal further enhancement of targeting (4 dB). The results showed potential of in vivo photoacoustic molecular imaging, providing a better guidance for diagnosis and treatment of cancer.
Subjects
Antibody; Gold nanorods; In vivo imaging; Molecular imaging; Multiple targeting; Photoacoustic imaging
SDGs
Other Subjects
Absorption; Acoustic waves; Cell culture; Cells; Chemotherapy; Drug delivery; Drug dosage; Drug therapy; Gold; Image enhancement; Light; Molecular structure; Molecules; Multiphoton processes; Nanoprobes; Nanorods; Nanostructures; Optics; Photoacoustic effect; Photons; Plasmons; Pulsed laser deposition; Ultrasonic applications; Ultrasonic transmission; Ultrasonics; Acousto optics; Anti cancer drugs; Cancer cells; Cell lines; Clinical outcome; Control Group (CON); Gold nanorods (GNR); Heterogeneous populations; In-vivo; laser irradiations; Molecular Imaging; Optical absorption (OA); Oral cancer; Peak absorption; Photoacoustic (PA) images; Simultaneous detection; surface molecules; target molecules; thermoacoustics; Treatment response; Two types; Ultrasonic imaging
Type
conference paper