A method for generating subtractive cDNA libraries retaining clones containing repetitive elements.
Resource
Nucleic Acids Research, 25(21), 4427-4428.
Journal
Nucleic Acids Research
Journal Volume
25
Journal Issue
21
Pages
4427-4428
Date Issued
1997
Date
1997
Author(s)
Lin, C.T.
Abstract
Here we describe a two-stepped photobiotin-based procedure to enrich a target (canine retinal) cDNA library for tissue specific clones without removing those containing repetitive (SINE) elements, despite the presence of these elements in the driver population. In a first hybridization excess SINE elements were hybridized to a driver (canine cerebellar) cDNA. In a second hybridization target cDNA was added to this reaction. The resulting cDNA library was enriched for retinal specific clones, but contained the same ratio of clones with SINE elements found in the unsubtracted library.
Type
journal article