Characterization of GABAergic Interneurons Within Locus Coeruleus Nucleus in Mice.
Date Issued
2014
Date
2014
Author(s)
Kuo, Zhao-chen
Abstract
The Locus coeruleus (LC) is a noradrenergic brainstem nucleus that is the major norepinephrine (NE) supply to the forebrain. It has been shown that GABA appears to be involved in the regulation of LC. Several studies have reported that extracellular GABA concentration is higher during REM/NREM sleep than wakefulness and that about two-third of boutons within LC contained GABA. Previous data of our lab showed that GABAB receptors could mediate tonic inhibition of LC neurons in rats, and this effect may link to ambient GABA concentration. Subcellular localization of GABAB receptors of LC nucleus in rats had been examined and found that most functional GABAB receptors are at extrasynaptic site, so ambient GABA could mediate tonic inhibition via extrasynaptic GABAB receptors here. However, the GABA source for tonic inhibition of LC neurons is still unclear. Many GABA afferents other brain nuclei have been reported previously, while recent studies found that local GABAergic interneuron may be a novel candidate of GABA supply to LC nucleus. However, the synaptic property and the role of LC GABAergic interneurons remain unknown. Thus we characterized these interneurons by using whole cell recording, following by staining of recorded interneurons for morphology reconstruction. Here we divided them into three group by their firing pattern and found some bouton-like structures on axons from the morphology reconstruction. But no functional pair has been found in the double patch experiments between LC neurons and GABAergic interneurons.
Subjects
藍斑核
中間神經元
伽瑪氨基丁酸
伽瑪氨基丁酸B型受體
穿透式電子顯微鏡
全細胞雙紀錄
Type
thesis
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