Applications of Fluorescence Spectra and Fluorescence Image in Evaluation of Rice Freshness
Date Issued
2008
Date
2008
Author(s)
Hsieh, Hao-Gu
Abstract
The objective of this project was to evaluate freshness of rice in different crop years using fluorescence spectrum and image. The paddy samples were obtained from different farmer’s associations. The experimental paddy was milled to obtain brown rice and white rice. The fluorescence responses of paddy rice, brown rice, and white rice were measured with Jasco FP-6300 Spectrofluorometer. The fluorescence responses were detected at wavelength in the range 400 nm to 600 nm after excitation at 365nm. The result shows that the aged white rice has stronger fluorescence response as compared to fresh sample. The white rice has strongest fluorescence response as compared to brown rice and paddy rice of the same crop year. In chemical analysis, this study used BTB-MR method to detect the pH value of rice. The coefficient of determination (R2) between the fluorescence peaks and pH value is 0.72.This result indicates that it is feasible to adopt fluorescence spectra to measure rice freshness.he fluorescence imaging system consists of four UV-A Hg lamps and a digital monochrome camera. The system is laboratory-based and is designed to be performed inside the darkened box. The fluorescence imaging system was tested for detecting rice in different crop year with respect to image response. The result shows that the white rice has strongest fluorescence image intensity as compared to brown rice and paddy rice in the same crop year. The significant difference in white rice fluorescence image intensity was found between the first and second crop of 2007. It is also feasible to adopt fluorescence imaging system to evaluate rice freshness.
Subjects
rice
freshness
fluorescence
spectra
image
Type
thesis
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