LC3 overexpression reduces Aβ neurotoxicity through increasing α7nAchR expression and autophagic activity in neurons and mice
Journal
Neuropharmacology
Journal Volume
93
Pages
243-251
Date Issued
2015
Author(s)
Abstract
Autophagy is an intracellular degradation pathway with dynamic interactions for eliminating damaged organelles and protein aggregates by lysosomal digestion. The EGFP-conjugated microtubule-associated protein 1 light chain 3 (EGFP-LC3) serves to monitor autophagic process. Extracellular β-amyloid peptide accumulation is reported as a major cause in Alzheimer's disease (AD) pathogenesis; large numbers of autophagic vacuoles accumulate in patients' brains. We previously demonstrated that extracellular Aβ (eAβ) induces strong autophagic response and α7nAChR acts as a carrier to bind with eAβ; which further inhibits Aβ-induced neurotoxicity via autophagic degradation. In the present study, we overexpressed LC3 in both neuroblastoma cells (SH-SY5Y/pEGFP-LC3) and mice (TgEGFP-LC3) to assess the effect of LC3 overexpression on Aβ neurotoxicity. SH-SY5Y/pEGFP-LC3 cells and primary cortical neuron cultures derived from E17 (embryonic day 17) TgEGFP-LC3 mice showed not only better resistance against Aβ neurotoxicity but also higher α7nAChR expression and autophagic activity than control. Administration of α-bungarotoxin (α-BTX) to block α7nAChR antagonized the neuroprotective action of SH-SY5Y/pECGF-LC3 cells, suggesting that eAβ binding with α7nAChR is an important step in Aβ detoxification. LC3 overexpression thus exerts neuroprotection through increasing α7nAChR expression for eAβ binding and further enhancing autophagic activity for Aβ clearance in vitro and in vivo. ? 2015 Elsevier Ltd. All rights reserved.
SDGs
Other Subjects
alpha bungarotoxin; amyloid beta protein; amyloid beta protein[1-42]; amyloid beta protein[25-35]; bungarotoxin receptor; membrane protein; microtubule associated protein 1 light chain 3; unclassified drug; actin; amyloid beta protein; amyloid beta-protein (1-42); amyloid beta-protein (25-35); bungarotoxin; bungarotoxin receptor; caspase 3; MAP1LC3 protein, mouse; microtubule associated protein; nicotinic receptor blocking agent; peptide fragment; animal cell; animal tissue; apoptosis; Article; autophagy; controlled study; disease resistance; embryo; gene overexpression; in vitro study; in vivo study; mouse; nerve cell; nerve cell culture; neuroprotection; neurotoxicity; nonhuman; priority journal; protein expression; protein protein interaction; animal; animal embryo; autophagy; brain cortex; cell culture; cytology; dose response; drug effects; gene expression regulation; genetics; human; metabolism; nerve cell; neuroblastoma; pathology; transgenic mouse; Actins; alpha7 Nicotinic Acetylcholine Receptor; Amyloid beta-Peptides; Animals; Autophagy; Bungarotoxins; Caspase 3; Cells, Cultured; Cerebral Cortex; Dose-Response Relationship, Drug; Embryo, Mammalian; Gene Expression Regulation; Humans; Mice; Mice, Transgenic; Microtubule-Associated Proteins; Neuroblastoma; Neurons; Nicotinic Antagonists; Peptide Fragments
Type
journal article