Anti-metastasis effect of phytochemicals on colorectal cancer cells via heparanase inhibitory activity
Date Issued
2011
Date
2011
Author(s)
Fu, Yi-Chien
Abstract
Heparanase is a type of endo-beta-D-glucuronidase which can cleave heparan sulfate (HS) chains both at the cell surface and in the extracellular matrix. The enzyme is secreted by metastatic tumor cells, destroys surrounding tissue, and alters the microenviroment of the cells. In this study, we used an in vitro cell model which is established by co-cultivation of cancer cells and the tumor microenvironment factors. Then, we investigated the effect of some phytochemicals on the metastasis of the cancer cells. The first part of this study used different concentration of PMA to induce differentiation of THP1 cells to polarized M2 macrophage, and found that 320 nM PMA increase 6.6% polarized M2 cell’s surface antibody. After that, we used the polarized M2 macrophage to induce HCT116 cell at different time points to establish the metastasis mode. We found that the induction occurred at 30 minute and 120 minute resulted in the highest degree of metastasis. In the second part of this study, we first investigated the effects of the some potential phytochemicals on the cell survival rate and
Subjects
heparanase
Tumor associated macrophage
metastasis cancer
SDGs
Type
thesis
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