Sequence Analysis and Serological Detection of Infectious Bronchitis Virus
Date Issued
2014
Date
2014
Author(s)
Tsai, Hsin-Fu
Abstract
Avian infectious bronchitis (IB) is a highly contagious viral disease of the chicken. Because IBV can change its genome by spontaneous mutation and genetic recombination easily, many strains emerge all over the world. This study investigated the prevalence and genotypes of IBVs in Taiwan recently. The samples of broilers were collected from poultry slaughter houses and the Taiwan Country chickens from IBV suspected case in the fields. IBV in samples were detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and grouped based on HVR1 in S1 sequence analysis. The result showed that the prevalence in poultry slaughter houses in Taiwan from November 2012 to January 2014 was 39.4% (26/66) with TW-II predominantly. The phylogenetic tree analysis showed that some Japan-like strains were detected besides Taiwan strains and vaccine strains. However, the N genes of those Japan-like strains were grouped into Taiwan strains. The 100-191 serum samples from fields were used to evaluate the relationship between HI test and a commercial ELISA kit. Base on HI result using 3263/04 as an antigen, the sensitivity and specificity of the commercial ELISA kit were 43.6% and 92.1%, respectively and 33.8% and 90.6%, respectively base on HI result using H120 as an antigen. The result of this study showed that the genetic variants of IBV have appeared in Taiwan.
Subjects
傳染性支氣管炎
台灣
序列分析
基因型
SDGs
Type
thesis
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