Effect of nitrogen concentration and form on the volatile composition of essential oil and growth of Melissa officinalis, M. officinalis ‘Quedlingburger’and M. officinalis ‘Citronella’
Date Issued
2008
Date
2008
Author(s)
Chou, Shih-Yao
Abstract
This research aims at the effect of nitrogen concentration and form to Melissa officinalis,M. officinalis ''Quedlingburger'' and M. officinalis ''Citronella'' on growth and volatile compound of leaf and essential oil, to establish a reasonable nitrogen application scope in production.. officinalis, M. officinalis ''Quedlingburger'' and M. officinalis ''Citronella'' were feed respectively by 7 level of nitrogen concentration solution (0 mM, 4 mM, 8 mM, 12 mM, 16 mM, 20 mM, 24 mM) continuously eight weeks, twice a week , each time 300 mL, the number of shoot and leaf all increased along with the nitrogen concentration increasing. The most number of shoots occurred by 20 mM treatment for M. officinalis, and by 24 mM treatment for M. officinalis ''Quedlingburger'' and M. officinalis ''Citronella''. The most number of leaf occurred by 16 mM treatment for M. officinalis and by 24 mM treatment for M. officinalis ''Quedlingburger'' and M. officinalis ''Citronella''.he most dry weight of aboveground part occurred by 20 mM treatment for M. officinalis and by 24 mM treatment for M. officinalis ''Quedlingburger'' and M. officinalis ''Citronella''. The most essential oil per unit fresh weight occurred by 16 mM treatment for M. officinalis and M. officinalis ''Citronella'', and by 8 mM treatment for M. officinalis ''Quedlingburger''. The most essential oil per unit dry weight occurred by 24 mM treatment for M. officinalis and M. officinalis ''Quedlingburger'' , and by 16 mM treatment for M. officinalis ''Citronella''.The composition of essential oil analysed by SPME and GC-MS showed that the main compounds of M. officinalis, M. officinalis ''Quedlingburger'' and M. officinalis ''Citronella'' were geranial and neral, approximately composed 89.53-98.48%, other main compounds were nerol, geraniol, geranyl formate, geranyl acetate and β-caryophyllene. . officinalis, M. officinalis ''Quedlingburge'' and M. officinalis ''Citronella'' were feed 4 weeks respectively by 20mM nitrate form nitrogen solution (NO3- - N) and ammonium form nitrogen solution (NH4 + - N) and urea nitrogen solution (Urea - N), the most number of leaf and shoot occurred by urea form nitrogen treatment for M. officinalis and the most number of leaf occurred by ammonium form nitrogen solution treatment for M. officinalis ''Quedlingburger'' and M. officinalis ''Citronella''he analysis of volatile compounds of leaf from M. officinalis, M. officinalis ''Quedlingburger'' and M. officinalis ''Citronella'' after different nitrogen form solution treatment by SPME and GC-MS showed that their main compounds were neral and geranial, and the total AI（Area index）value dropped along with treatment time, and started to rise 2 weeks later.n correlation analysis between SPAD reading and the ethanol extractable chlorophyll, the correlation coefficient respectively was R2=0.94, R2=0.97 for M. officinalis by the ammonium form nitrogen solution treatment and by the nitrate form nitrogen solution treatment, and the correlation coefficient respectively was R2=0.91, R2 =0.96 for M. officinalis ‘Quedlingburger'' and M. officinalis ''Citronella'' by nitrate form nitrogen solution treatment. While the correlation didn’t reach significant level for three kind of M. officinalis by urea nitrogen solution treatment
Subjects
essential oil
citral
nitrate form nitrogen
ammounium form nitrogen
Type
thesis
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