Composition and activity of n2-fixing microorganisms in mangrove forest soils
Journal
Forests
Journal Volume
12
Journal Issue
7
Date Issued
2021
Author(s)
Abstract
Mangrove forests are considered to be a highly productive ecosystem, but they are also generally nitrogen (N)-limited. Thus, soil N2 fixation can be important for the stability of both mangrove ecosystem functions and upland N supply. This study evaluates the N2 fixation activity and composition of relevant microorganisms in two coastal mangrove forests—the Guandu mangrove in an upstream estuary and the Bali mangrove in a downstream estuary—using the acetylene reduction method, real-time polymerase chain reaction, and next-generation sequencing. The results demonstrated that ambient nitrogenase activity was higher in downstream mangrove forests (13.2–15.6 nmol hr?1 g?1 soil) than in upstream mangrove forests (0.2–1.4 nmol hr?1 g?1 soil). However, both the maximum potential nitrogenase activity and nitrogenase gene (nifH gene) copy number were found to be higher in the upstream than in the downstream mangrove forests, implying that the nitrogenase activity and diazotrophic abundance may not necessarily be positively correlated. In addition, amended MoO4 (which inhibits the activity of sulfate-reducing bacteria in N2-fixation) yielded low nitrogenase activity, and sulfate-reducing bacteria made up 20– 50% of the relative diazotrophic abundance in the mangrove forests, indicating that these bacteria might be the major active diazotrophs in this environment. ? 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Subjects
Acetylene reduction
Diazotrophs
Mangrove
N2 fixation
NifH gene
Sulfate reducing bacteria
Ecosystems
Forestry
Genes
Molybdenum compounds
Polymerase chain reaction
Soils
Sulfur compounds
Mangrove ecosystems
Mangrove forest
Next-generation sequencing
Nitrogenase activity
Nitrogenase genes
Real time polymerase chain reactions
Reduction method
Bacteria
forest soil
gene expression
mangrove
microbial community
nitrogen fixation
sulfate-reducing bacterium
Molybdenum Compounds
Sulfur Compounds
Guandu Wetland
Taiwan
Bacteria (microorganisms)
Type
journal article