Comprehensive evaluation of cryopreserved aortic homografts: Long-term mechanical and histological preservation across human and porcine models
Journal
Journal of Tissue Engineering
Series/Report No.
Journal of Tissue Engineering
Journal Volume
16
ISSN
2041-7314
2041-7314
Date Issued
2025-11
Author(s)
Liao, Hsien-Chi
Tsou, Kuan-Chuan
Wu, Ying-Syuan
Su, Wei-Ching
Ju, Yu-Ten
Abstract
This study aimed to examine the histological and mechanical effects of cryopreservation on human aortic tissues, focussing on storage duration and conditions. Assessments included smooth muscle cell integrity, elastic fibre preservation, and endothelial viability. Cryopreservation with dimethyl sulphoxide (DMSO) significantly reduced smooth muscle cell nuclei loss and maintained elastic fibre integrity. However, elastic fibre thickness increased after 12 months. Isolectin B4 staining showed reduced endothelial cell viability across all groups. No significant changes were observed in mucoid extracellular matrix accumulation or elastic fibre fragmentation. These findings suggest that cryopreservation with DMSO effectively maintains structural integrity for up to 12 months but requires refinement to address endothelial and biomechanical concerns. Cryopreserved aortic allografts demonstrated structural and functional performance when stored at low temperatures, confirming their viability for reconstructive surgeries. The study highlights the importance of the timely utilisation of cryopreserved grafts and optimising preservation techniques to advance surgical applications.
Subjects
aortic homografts
biomaterial
cryopreserved aorta
tracheal regeneration
SDGs
Publisher
SAGE Publications
Type
journal article