The clinical implications of MMP-11 and CK-20 expression in human breast cancer
Journal
Clinica Chimica Acta
Journal Volume
411
Journal Issue
3/4
Pages
234-241
Date Issued
2010
Author(s)
Abstract
Background: Tumor invasiveness and metastasis in cancer progression is manifested by epigenetic abnormality. However, it remains unknown whether transcription regulation of matrix metalloproteinase-11(MMP-11) and cytoskeleton-20 (CK-20) genes for the homoeostasis of epithelial/connective interface that can enhance cell dissemination and invasion may act as alternative mutators to tumor clinicopathology. Methods: Paired cancerous and tumor-adjacent normal tissues from 72 breast cancer patients were assayed for the expression of MMP-11 and CK-20 by using real-time RT-PCR. The expression profiles were evaluated for the association with clinicopathological factors. Results: Breast tumor tissues displayed higher expression levels of MMP-11 and CK-20 than those of the adjacent non-cancerous tissues. Overexpression of either MMP-11 or CK-20 correlated with patients having poorly differentiated tumors (PMMP-11 = 0.01 and PCK-20 = 0.05) and lymph node metastasis (LNM) (PMMP-11 = 0.004 and PCK-20 = 0.001). A synergistic effect between MMP-11 and CK-20 on risk elevation was significant in patients with advanced tumor stage (OR = 2.03, 95%CI = 1.10-3.77) and LNM (OR = 2.83, 95%CI = 1.20-4.71). Additionally, patients lacking progesterone receptor exhibited high expression of MMP-11 and CK-20. Conclusion: We demonstrate that MMP-11 and CK-20 are probable prognostic markers whose expression reflects the stages of tumor differentiation and LNM of breast cancer. ? 2009 Elsevier B.V. All rights reserved.
SDGs
Other Subjects
cytokeratin 20; estrogen receptor; messenger RNA; progesterone receptor; stromelysin 3; adult; age; aged; article; breast cancer; cancer staging; cell spreading; clinical feature; controlled study; disease association; family history; female; gene expression; genetic epigenesis; homeostasis; human; human tissue; immunohistochemistry; immunoreactivity; lymph node metastasis; major clinical study; priority journal; real time polymerase chain reaction; risk factor; transcription regulation; tumor volume; Adult; Aged; Antibodies; Breast Neoplasms; Female; Gene Expression Regulation, Neoplastic; Genetic Predisposition to Disease; Humans; Immunohistochemistry; Keratin-20; Lymphatic Metastasis; Matrix Metalloproteinase 11; Middle Aged; Neoplasm Staging; Prognosis; Receptors, Estrogen; Receptors, Progesterone; RNA, Messenger; Tumor Markers, Biological
Type
journal article