Functional Characterization of AtLecRK-VI.2 in Nicotiana benthamiana and Tomato Plants and Screening of Cysteine-rich Receptor-like Kinases Involved in Arabidopsis Defense Responses
Date Issued
2012
Date
2012
Author(s)
Huang, Pin-Yao
Abstract
Recognition of pathogen-associated molecular patterns (PAMPs) by host cell
surface-localized pattern-recognition receptors (PRRs) activates plant innate
immunity. PAMP-triggered immunity (PTI) is the first layer of plant immunity.
Crucial experiments demonstrated that the transfer of the Arabidopsis PRRs EFR to N.
benthamiana and tomato (S. lycopersicum) gave the transgenic plants broad-spectrum
resistance against pathogens, suggesting PTI signaling machinery is conserved across
different plant families and setting an example utilizing PTI to confer disease
resistance in crops. We have recently identified Arabidopsis LecRK-VI.2 as a positive
regulator of PTI. To test whether AtLecRK-VI.2 can also be a positive regulator of
PTI in other plant species, I generated transgenic N. benthamiana and tomato plants
over-expressing AtLecRK-VI.2. Preliminary test on transgenic N. benthamiana T2
plants showed that they are resistant against bacteria P. syringae pv. syringae (Pss)
B728a. Additional analyses suggest their resistance is due to enhanced PTI responses,
such as callose deposition, ROS production, and expression of PTI marker genes.
These preliminary findi ngs indicate N. benthamiana contains the components required
for AtLecRK-VI.2 signaling to positively modulate the PTI response.
Of the receptor-like kinases (RLKs) in Arabidopsis, cysteine-rich receptor-like kinases (CRKs) have been suggested to play important roles in the regulation of pathogen defense. Combining transcriptome analysis from a AtLecRK-VI.2 overexpression line and publicly available microarray data revealed 12 CRKs that could potentially be involved in plant defense. Our results show that loss-of-function analysis on these CRKs did not reveal an altered phenotype; thus further analysis is being conducted using gain-of-function analysis.
Subjects
Arabidopsis
Nicotiana benthamiana
PTI
transgenic plants
lectin receptor-like kinase
defense response
bacteria
cysteine-rich receptor-like kinase
lectin receptor-like kinase-VI.2
Type
thesis
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