A Modified Procedure for Caseinophosphopeptide Analysis
Journal
Journal of Chromatographic Science
Journal Volume
34
Journal Issue
8
Pages
358-361
Date Issued
1996
Author(s)
Lin Jr. H.
Abstract
A modified procedure is established for analyzing caseinophosphopeptides. The sodium casemate hydrolysate is first treated by immobilized metal ion affinity chromatography to enrich the phosphopeptides. Because of the formation of Fe3+-peptide complexes, ethylenediaminetetraacetic acid is added to the bound fraction eluted with the immobilized metal ion affinity chromatography to disintegrate the complexes. Thus, the subsequent high-performance liquid Chromatographic analysis is facilitated. A stepwise gradient elution is also suggested to enhance the resolution of caseinophosphopeptides during high-performance liquid chromatographic analysis.
Type
journal article
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