Thermosensitive, injectable, biodegradable, triblock copolymer hydrogel as carriers for camptothecin
Date Issued
2009
Date
2009
Author(s)
Liang, Kuo-Sheng
Abstract
In this study, we use a biodegradable monomer (ε-Caprolactone) to combine with a biodegradable polymer (polyehylene glycol) and a biodegradable triblock copolymer (PCL-PEG-PCL) was synthesis. The triblock copolymer (PCL–PEG–PCL) is thermosensitive when weights of those monomers were combined at some special ratio and its aqueous solution can undergo the sol–gel–sol transition as the temperature increases. The hydrogel was fully biodegradable and biocompatible. GPC and NMR 1H are used for characterizing the polymer. Thermal stability was tested by TGA. Chemotherapy drugs：CPT-11 and SN-38 were loaded into hydrogel made by triblock copolymers at several concentrations. Then, a scanning electron microscope was used to observe morphology of hydrgel loaded with chemotherapy drugs. The morphology of hydrogel loaded with hydrophilic drug CPT-11 is different from the polymer concentrations and hydrogel loaded with hydrophobic drug SN-38 show another appearance under the same polymer concentration with CPT-11. In vitro drug release from thermosensitive hydrogel was investigated. CPT-11 was released within two days from hydrgel when they are loaded at a high concentration (5mg/ml). The releasing curve of hydrogel loaded with SN-38 at the same concentration shows a slowly stable and linear trend within 10 days. In vivo experiments of CPT-11 indicated that the treatment using hydrogel loaded with 5 doses (inject once) for suppressing tumor growth is the same effective as five injections and the toxicity is lower. In the experiments of SN-38, hydrogel loaded with 5 doses also shows a lower toxicity than five injections. Thus, they can be used as a suitable drug - polymer implant for local release of chemotherapeutic drug like irinotecan or SN-38.
Subjects
thermosensitive
biodegradable
triblock copolymers
camptothecin
drug delivery
injectable hydrogel
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-98-R96548032-1.pdf
Size
23.53 KB
Format
Adobe PDF
Checksum
(MD5):fd0143b1f4f2f127acb8169caa287396