Interleukin-6 and Interleukin-8 Induced by Taipei City Air Pollution Particulate Matter in Vitro
Date Issued
2007
Date
2007
Author(s)
Chiang, Yi-Ching
DOI
zh-TW
Abstract
This study was intended to look at the induction of the proinflammatory cytokines（interleukin 6 and interleukin 8）while the human bronchial epithelial cells （BEAS-2B cell）were exposed to air pollution particulate matters and their particulate-bond polynuclear aromatic hydrocarbons（PAHs）from vehicles and diesel exhaust. Field samples containing ambient particulate matters were collected using MOUDI impactor in the campus of each two elementary schools at one area where traffic condition was classfied as the heavy, the moderate or the light.
The solvent extracts of the field samples were divided into two parts in which one part was treated with the microsyringe filter. The results demonstrated that BEAS-2B cells produced IL-6 and IL-8 due to exposure to the solvent extract matters with PAHs. Upon the trials of Taguchi 's orthogonal array where the standard PAHs were added to the solvent extract matter, indicated that PAHs concentration in terms of toxic equivalency quantity（TEQ）was the statistically significant variable. The IL-8 concentration increase with increment of TEQ（p < 0.05）. However, the field samples without standard addition of PAHs into the solvent extract matter demonstrated the effect of TEQ and particulate on the induction of IL-6 and IL-8. The cells seemly keep in homeostasis as they exposure to samples with relatively great particulate matter and lower PAH TEQ and then the reaction mode alter and move toward the phase of proinflammation when PAH TEQ rise up to a certain level. In regard to particle mass, its influence on release of cytokines may exist according to parts of implication from this study. This study gives implication that ambient particulate matter with particulate-bond polynuclear aromatic hydrocarbons may induce respiratory diseases that involve in the inflammation of epithelial cells, but mechanism involved needs further study.
Subjects
微粒
多環芳香烴化合物（polynuclear aromatic hydrocarbons, PAHs）
第六介白素（interleukin-6）
第八介白素（interleukin-8）
particulate matter
polynuclear aromatic hydrocarbons（PAHs）
interleukin 6
interleukin 8
Type
thesis
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