菜鴨因年齡及選拔造成蛋殼品質下降之機制(3/3)
Date Issued
2003
Date
2003
Author(s)
沈添富
DOI
912313B002371
Abstract
The objectives of this study were to
investigate the mechanism of deterioration
of eggshell quality associated with age and
selection. The 1st generation (F1) and 2nd
generation (F2) of Tsaiya ducks laying
high eggshell strength (HES) and low
eggshell strength (LES) were produced
through selection. Trial 1: When F1
ducks reached 27, 49, and 71 weeks of age,
egg weight, eggshell strength, and eggshell
thickness were collected. Eggs collected
were used to analyze the composition of
eggshell, including contents of calcium,
magnesium, and organic matrix. Proteins
in the eggshell were also analyzed and
compared by electrophoresis. Trial 2:
The blood and uterine fluid of F2 ducks at
28, 49, and 71 weeks of age were collected
to compare pH, Ca and Mg concentrations
in the blood and uterine fluid between
HES and LES ducks. In addition, volume
of uterine fluid and proteins in the uterine
fluid were also studied.
The results showed that eggshell
strength of eggs from HES and LES were
significantly different for both generations.
The eggshell calcium content was similar
between HES and LES populations.
The significantly higher eggshell Mg
content in the LES duck eggshell was
observed throughout this study. Organic
matrix contents in the eggshell of HES and
LES were similar, but they tended to
increase with age. The molecular
weights of eggshell proteins were between
6.5 and 200 KDa as determined by
electrophoresis. Two proteins of them,
80 and 180 KDa, appeared to be associated
with eggshell quality. The eggshell of
71-week-old ducks seemed to have more
180 kDa protein than that of 27-week-old
ducks. Two proteins in the duck eggshell
were able to show cross-reaction with
hen’s anti-ovocleidin-17 and
anti-ovocleidin-116, respectively. Eight
amino acid residues of NKYPKGWL were
obtained through N-terminal sequencing of
the 15 kDa protein in the duck eggshell.
The HES ducks had a trend of higher
blood pH than the LES ones. However,
the LES ducks had higher levels total
blood calcium levels. The tendencies for
blood pH and total calcium were similar in
the three ages. Total blood magnesium
levels were similar between HES and LES
ducks. In respect of uterine fluid, pH and
total magnesium levels were similar
between HES and LES ducks. However,
total calcium concentrations were higher in
the HES ducks at some time points than in
the LES ones. A trend of higher uterine
fluid volume was observed in the HES
ducks in three ages. Two proteins in the
duck’s uterine fluid were able to show
cross-reaction with hen’s
anti-ovocleidin-17 and anti-ovocleidin-116,
which means non-specific binding existing.
SDS-PAGE of proteins in uterine fluid
showed protein profiles were different
among different time points. No
significant differences were observed in
proteins of uterine fluid between HES and
LES ducks based on the SDS-PAGE
results. Some eggshell strength-specific
bands were observed through differential
display reverse transcription-polymerase
chain reaction. Two of them had been
cloned and sequenced.
This study has demonstrated the
difference of contents in the eggshell,
blood, and uterine fluid of HES and LES
ducks. Furthermore, gene expression of
shell gland mucosa of HES and LES ducks
has also been studied. These data would
be helpful to provide some information in
solving the problems of eggshell quality
deterioration.
Subjects
Tsaiya duck
Eggshell strength
Calcium
Magnesium
Protein
Gene expression
Publisher
臺北市：國立臺灣大學動物科學技術學系暨研究所
Type
report
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