The study of metal particles and releasing ions inducing cell death on J774 macrophages
Date Issued
2006
Date
2006
Author(s)
Huang, Wei-Chih
DOI
zh-TW
Abstract
Most of the total joint prosthesis used currently are made of metal base and ultra-high molecular weight polyethylene （UHMWPE）. Wear debris from UHMWPE （found in the artificial joint implants） may easily cause osteolysis, which may lead to the loosening of the implant and, hence, failure of prosthesis.
The materials used in the researches in the past on simulating wear particles from the joint prosthesis were mostly pure metal particles, but there were a lot of differences between the real compositions of joint prosthesis and pure metal particles. This study used the Co-Cr-Mo alloy particles created by rotational electrode process, cobalt, nickel pure metal particles and cobalt, nickel chemical compound as materials, and cultured J774 murine macrophage with these materials for examine the biocompatibility.
As a result, Co-Cr-Mo alloy particles created by rotational electrode process, cobalt, nickel pure metal particles and cobalt, nickel chemical compound all cause cytotoxicity to the cells, but the Co-Cr-Mo alloy particles were less cytotoxic to cobalt, nickel pure metal particles. And we found that cobalt, nickel pure metal particles would release ions in solutions, we supposed that the reason of the death of the cells made by metal particles includes the cytotoxicity created by the release of ions.
This study produced the Co-Cr-Mo alloy particles successfully by rotational electrode process, to simulate the wear particles from the joint prosthesis. It provides an alternative way to experiment in relative researches in the future.
Subjects
工關節成形術
旋轉電極法
磨耗顆粒
鈷鉻鉬和鈷鎳顆粒
生物相容性
total joint prosthesis
rotational electrode process
wear particles, CoCrMo particles
cobalt particles
nickel particles
biocompatibilities
Type
thesis