Facilitating tumor spheroid-based bioassays and: In vitro blood vessel modeling via bioinspired self-formation microstructure devices
Journal
Lab on a Chip
Journal Volume
18
Journal Issue
16
Pages
2453-2465
Date Issued
2018
Author(s)
Kuo, C.-T.
Lu, S.-R.
Chen, W.-M.
Wang, J.-Y.
Lee, S.-C.
Wo, A.M.
B. P. C. Chen
Abstract
Non-planar microstructure-based tissue culture devices have emerged as powerful tools to mimic in vivo physiological microenvironments in a wide range of medical applications. Here we report a spontaneous aqueous molding approach-inspired by Stenocara gracilipes beetles-to rapidly fabricate non-planar microstructure devices for facilitating tissue-based bioassays. The device fabrication is determined from the self-assembled liquid morphology, which is induced by condensation or guided by surface tension. Through experiments and modeling, we reveal that the molding mainly comprises two typical circular and striped domains, highlighting versatile applications for bioengineering. In addition, the molding characteristic is dependent on the geometry of the patterned wetting surfaces, the working volume of the liquid, and the interaction between the liquid and the substrate. The theoretical model, based on the geometry of the patterned liquid, is highly consistent with experimental data. We also demonstrate that our approach can facilitate the culturing of tumor spheroids incorporated with biomimic nano-cilia, rapid high-throughput drug screening, tumor spheroid migration assay, and in vitro modeling of blood vessels. Remarkably, the delivery of multiple concentrations of drugs and their associate mixtures (a total of 25 test spots in one device) can be carried out simultaneously within seconds. Taken together, these insights may offer new opportunities to tailor non-planar microstructures, and our proposed methodology can be applicable for the emerging needs in tumor cell biology and tissue engineering. ? 2018 The Royal Society of Chemistry.
SDGs
Other Subjects
adenosine triphosphate; benzyloxycarbonylleucylleucylleucinal; cisplatin; F actin; hypoxia inducible factor 1alpha; sphingosine 1 phosphate; Article; assay; bioassay; blood vessel; blood vessel function; cancer resistance; comparative study; concentration response; contact angle; DNA repair; drug potentiation; drug screening; geometry; human; human cell; in vitro study; metastasis; microtechnology; priority journal; surface property; surface tension; theoretical model; tissue engineering; tumor cell; tumor cell culture; tumor spheroid; umbilical vein endothelial cell; vascularization; animal; beetle; bioassay; biomimetics; blood vessel; cell motion; devices; drug effect; drug screening; lab on a chip; multicellular spheroid; pathology; pathophysiology; tumor cell line; Animals; Biological Assay; Biomimetics; Blood Vessels; Cell Line, Tumor; Cell Movement; Coleoptera; Drug Screening Assays, Antitumor; Humans; Lab-On-A-Chip Devices; Spheroids, Cellular
Publisher
Royal Society of Chemistry
Type
journal article