Stabilizer-free poly(lactide-co-glycolide) nanoparticles for multimodal biomedical probes
Journal
Biomaterials
Journal Volume
29
Journal Issue
13
Pages
2104 - 2112
Date Issued
2008-05
Author(s)
Fong-Yu Cheng
Saprina Ping-Hsien Wang
Chia-Hao Su
Tsung-Liu Tsai
Ping-Ching Wu
Dar-Bin Shieh
Patrick Ching-Ho Hsieh
Chen-Sheng Yeh
Abstract
Apart from the reported PLGA submicro- and microspheres with broad size distribution, we have successfully developed a methodology using nanoprecipitation to prepare different sizes of PLGA nanoparticles with narrow size distributions. The newly developed PLGA nanoparticles could be readily modified with hydrophilic biomaterials on their surface and entrap hydrophobic drugs into their interiors. The encapsulation of FITC inside PLGA nanoparticles displayed a controlled release of drug system. The surfaces of the FITC entrapped PLGA nanoparticles were conjugated with quantum dots to serve as bimodal imaging probes. For nuclear transport, combination of nuclear localization signal (NLS) and PLGA nanoparticles, PLGA nanoparticles could successfully enter into HeLa cells nuclei. From tissue uptake results, PLGA nanoparticles had more uptaken by brain and liver than other tissues. The iron oxide nanoparticles-conjugated PLGA nanoparticle showed high efficiency of relaxivities r2 and could be used as the powerful magnetic resonance imaging (MRI) agents. © 2008 Elsevier Ltd. All rights reserved.
Subjects
Drug delivery; MRI; Nanoparticles; Nuclear localization signal; PLGA
Other Subjects
Biomaterials; Drug delivery; Hydrophilicity; Particle size analysis; Precipitation (chemical); Size distribution; Hydrophilic biomaterials; Multimodal biomedical probes; Nanoprecipitation; Nuclear localization signal; Nanoparticles; biomaterial; iron oxide; nanoparticle; polyglactin; quantum dot; article; controlled study; drug delivery system; encapsulation; human; human cell; hydrophilicity; hydrophobicity; molecular probe; nuclear localization signal; nuclear magnetic resonance imaging; priority journal; transmission electron microscopy; Animals; Biomedical Engineering; Hela Cells; Humans; Lactic Acid; Magnetic Resonance Imaging; Microscopy, Electron, Transmission; Nanoparticles; Organ Specificity; Polyglycolic Acid; Rats; Rats, Sprague-Dawley; Tissue Culture Techniques
Type
journal article