Molecular characterization of class 1 integrons and antimicrobial resistance in Aeromonas strains from foodborne outbreak-suspect samples and environmental sources in Taiwan
Resource
Diagnostic Microbiology and Infectious Disease 59 (2): 191-197
Journal
Diagnostic Microbiology and Infectious Disease
Pages
191-197
Date Issued
2007
Date
2007
Author(s)
Chang, Yu-Chang
Shih, Daniel Yang-Chih
Wang, Jan-Yi
Yang, Shang-Shyng
Abstract
One hundred thirty-three Aeromonas spp. isolates were examined for multiple antibiotic resistance phenotypes and prevalence of class 1 integron sequences. Twenty-four (18.0%) of these isolates contained class 1 integron. Seven different class 1 integrons were found among 24strains, with a total of 10 different gene cassettes encoding for resistance to trimethoprim (dfr12 and dfr2d), aminoglycosides (aadA1 and aadA2), β-lactam antibiotics (oxa2), chloramphenicol (catB3 and catB8), quaternary ammonium amines (qacE2), and 2 ORFs (orfD and orfF) with unknown function. Rate of antibiotic resistance was different between integron-positive and integron-negative strains. Trimethoprim and trimethoprim-sulphamethoxazole resistances were commonly associated with integron, and all of integron-positive isolates were multiple resistant to more than 3 agents. Resistance to as many as 10 antimicrobial agents were observed in integron-positive strains. Several cassette arrays of class 1 integrons identified in this study were not previously reported in Aeromonas strains. This study demonstrates the wide distribution of class 1 integron in Aeromonas spp. isolated from foodborne outbreak-suspect samples and environmental sources in Taiwan. ? 2007 Elsevier Inc. All rights reserved.
Subjects
Aeromonas spp.; Antibiotic resistance; Integron; Multiple resistance
SDGs
Other Subjects
amikacin; aminoglycoside derivative; ampicillin; beta lactam antibiotic; cefazolin; ceftriaxone; chloramphenicol; cotrimoxazole; gentamicin; kanamycin; nalidixic acid; quaternary ammonium derivative; streptomycin; sulfamethoxazole; tetracycline; trimethoprim; aadA1 gene; aadA2 gene; Aeromonas; Aeromonas encheleia; Aeromonas hydrophila; Aeromonas punctata; Aeromonas sobria; Aeromonas veronii; antibiotic resistance; article; bacterial strain; bacterium isolate; catb3 gene; catB8 gene; controlled study; dfr12 gene; dfr2d gene; epidemic; food contamination; gene; gene cassette; gene sequence; integron; nonhuman; nucleotide sequence; open reading frame; orfD gene; orfF gene; oxa2 gene; phenotype; prevalence; priority journal; qacE2 gene; Taiwan; Aeromonas; Animals; Disease Outbreaks; Drug Resistance, Multiple, Bacterial; Environmental Microbiology; Food Poisoning; Gram-Negative Bacterial Infections; Humans; Integrons; Molecular Sequence Data; Sequence Analysis, DNA; Taiwan
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