在豬隻心房顫動模式中功能性基因體的變化(2/3)
Other Title
Functional Genomic Studies in a Porcine Model of Atrial Fibrillation(2/3)
Date Issued
2004
Date
2004
Author(s)
賴凌平
DOI
922314B002213
Abstract
There are two specific aims in the present
study:
1. To establish a porcine model of atrial
fibrillation.
2. To study the changes of atrial tissue from
a viewpoint of function genomics by2-D
PAGE and DNA microarray techniques.
This project is a three-year project.
During the period between August 2002 and
now, we have already accomplished the
followings
1. To establish a porcine model of atrial
fibrillation by rapid atrial pacing. The
atrial fibrillation was observed after rapid
pacing for 4 weeks and was incessant
until sacrifice.
2. We have established the techniques of
cDNA microarray to profile the gene
expression pattern in porcine atrial tissue.
3. We have successfully used the 2D-PAGE
techniques to separate protein spots in
atrial tissue. These protein spot include
both known and unknown protein spots.
4. We produced sham pigs by implanting
the pacemaker without pacing.
5. We used the cDNA microarray technique
to compare the difference between the
gene expression pattern between atrial
fibrillation and control pigs.
6. We used the 2D-PAGE technique to
compare the protein spot pattern between
atrial fibrillation and control pigs.
In the future year, we will accomplish the
following items
1. We will compare the mRNA change and
protein change. Significant findings will be re-confirm by Northern and Western
blotting techniques.
2. We will write a manuscript and submit it
to an SCI journal.
With the present study, we expect to
identify crucial changes in the development
of atrial fibrillation. This information will
contribute to future development of treatment
for atrial fibrillation.
Subjects
atrial fibrillation
microarray
2D-PAGEl
Publisher
臺北市：國立臺灣大學醫學院內科
Type
report
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