Studies on Radiation Resistant Rubrobacter spp. from geothermal areas in Taiwan
Date Issued
2007
Date
2007
Author(s)
Tsai, Hsin-Lun
DOI
en-US
Abstract
In the previous studies, several genera of thermophilic bacteria with radiation resistance were isolated from geothermal environments in Taiwan by our laboratory. Though Rubrobacter spp. exhibited extreme radiation resistance, but only a few species had been isolated and studied. According to 16 S rRNA gene sequence analysis, 32 strains of thermophiles belong to genus Rubrobacter were isolated and partially characterized from ten geothermal areas in Taiwan.
In this study, we focus on two groups containing six isolates that had the potential to be new species in genus Rubrobacter for further characterization and identification. These new isolates were Gram positive thermophiles with the ability to resist radiation. According to genetic, morphological, physiological and biochemical properties, group 1 isolates NTU-806, NTU-807, and NTU-808 from Danda were identified as R. taiwanensis. Group 2 isolates NTU-829, NTU-1106, and NTU-1119 showed 94 % 16S rRNA gene sequence similarities and 26-29% DNA-DNA similarities to R. radiotolerans. These three isolates grew optimally at 50 oC, and in the medium containing 0-7 % (w/v) NaCl, while R. radiotolerans at 45 oC and 0-3% NaCl. The new isolates from group 2 contained higher amount of 18:0 anteiso and 19:0 anteiso fatty acids than R. radiotolerans. Based on DNA homology, 16S rRNA gene sequence, as well as physiological and biochemical analyses, strains NTU-829, NTU-1106, and NTU-1119 could be identified as a novel species of genus Rubrobacter.
Subjects
抗輻射
嗜熱菌
溫泉
Rubrobacter
Radiation resistance
Thermophiles
Type
other
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