Effect of Induced Pluripotent Stem Cells on Experimental Light-Induced Retinal Degeneration in Rats
Date Issued
2010
Date
2010
Author(s)
Yueh, Mei-Hsuan
Abstract
Light-induced retinal degeneration in a rat model was used to mimic the visual cell apoptosis of retinal degenerations. Recent reserchs found that transplantation of mesenchymal stem cells improves repair and functional recovery of myocardial infarction swine. The possible hyposis is that mesenchymal stem cells could achieve protection through release mediators rather than direct cardiac regeneration. Therefore, we aim to study whether induced-pluripotent stem cells (iPS cells) possess protect effects on degenerative retina in rats. iPS cells (10000 cells/μl, 100000 cells/μl, 1000000 cells/μl; 0.01ml) or vehicle (PBS; 0.01 ml) were injected intravitreally in Sprague-Dawley rats 30 minutes after exposure to 12000 lux of white light for two hours. The retinal function of the test rats were assessed by measuring the electroretinogram (ERG) before light damage and at 24 hours, 48 hours, and 96 hours, and seven days after light damage. The amplitude of a wave and b wave after light damage divided by the amplitude of a wave and b wave before light damage is equal to a wave and b wave residual percentage. The retinal sections were evaluated histologically and thickness of retina and outer nuclear layer (ONL) were measured. The residual percentage of retinal function and retinal thickness were statistically analysed by ANOVA.
Results showed that the residual ratio of ERG were significantly higher in the iPS-cells-treated group (n=12) than the control (n=8) and vehicle-treated (n=8) group, respectively. Retinal sections revealed that the ONL of the retina were preserved in the iPS-cells-treated group, and the thickness of retina and ONL in iPS-cells-treated group (n=3) was thicker than the control (n=3) and vehicle-treated group (n=3). Thus, the iPS cells provided protective effect in light induced retinal degenerative rats.
Subjects
Induced-pluripotent stem cells
iPS cells
stem cells
Light induced retinal degeneration
Canine inherited retinal degeneration
Photodamage
Type
thesis
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