Monascus-fermented yellow pigments monascin and ankaflavin showed antiobesity effect via the suppression of differentiation and lipogenesis in obese rats fed a high-fat diet
Journal
Journal of Agricultural and Food Chemistry
Journal Volume
61
Journal Issue
7
Pages
1493-1500
Date Issued
2013
Author(s)
Abstract
Monascus-fermented monascin and ankaflavin are found to strongly inhibit differentiation and lipogenesis and stimulate lipolysis effects in a 3T3-L1 preadipocyte model, but the in vivo regulation mechanism is unclear. This study uses obese rats caused by a high-fat diet to examine the effects of daily monascin and ankaflavin feeding (8 weeks) on antiobesity effects and modulation of differentiation, lipogenesis, and lipid absorption. The results show that monascin and ankaflavin had a significant antiobesity effect, which should result from the modulation of monascin and ankaflavin on the inhibition of differentiation by inhibiting CCAT/enhancer-binding protein β (C/EBPβ) expression (36.4% and 48.3%) and its downstream peroxisome proliferator- activated receptor γ (PPARγ) (55.6% and 64.5%) and CCAT/enhancer-binding protein α (C/EBPα) expressions (25.2% and 33.2%) and the inhibition of lipogenesis by increasing lipase activity (14.0% and 10.7%) and decreasing heparin releasable lipoprotein lipase (HR-LPL) activity (34.8% and 30.5%). Furthermore, monascin and ankaflavin are the first agents found to suppress Niemann-Pick C1 Like 1 (NPC1L1) protein expression (73.6% and 26.1%) associated with small intestine tissue lipid absorption. Importantly, monascin and ankaflavin are not like monacolin K, which increases creatine phosphokinase (CPK) activity, known as a rhabdomyolysis indicator. ? 2013 American Chemical Society.
Subjects
ankaflavin; monacolin K; monascin; Monascus; obesity
SDGs
Other Subjects
Ankaflavin; Monacolin k; Monascin; Monascus; obesity; Cytology; Modulation; Proteins; Rats; Tissue; Nutrition; ankaflavin; antiobesity agent; carrier protein; CCAAT enhancer binding protein alpha; CCAAT enhancer binding protein beta; Cebpb protein, rat; creatine kinase; fused heterocyclic rings; mevinolin; monascin; NPC1L1 protein, rat; peroxisome proliferator activated receptor gamma; riboflavin derivative; adipocyte; animal; article; cell differentiation; chemistry; dose response; drug effect; fermentation; genetics; lipid diet; lipogenesis; lipolysis; male; metabolism; Monascus; obesity; rat; Sprague Dawley rat; Adipocytes; Animals; Anti-Obesity Agents; CCAAT-Enhancer-Binding Protein-alpha; CCAAT-Enhancer-Binding Protein-beta; Cell Differentiation; Creatine Kinase; Diet, High-Fat; Dose-Response Relationship, Drug; Fermentation; Flavins; Heterocyclic Compounds, 3-Ring; Lipogenesis; Lipolysis; Lovastatin; Male; Membrane Transport Proteins; Monascus; Obesity; PPAR gamma; Rats; Rats, Sprague-Dawley
Type
journal article