Fast Fourier transform second harmonic generation microscopy for probing the 3D structure of corneal lamella
Journal
Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Journal Volume
10882
Date Issued
2019
Author(s)
Abstract
Cornea is one of the collagen-rich connective tissues and plays an important role in vision. While X-ray scattering techniques are able to determine bulk structure of cornea, second harmonic generation microscopy can reveal depthdependent details of the corneal stroma comprised of a layered network of fibrillar collagen. In this work, we used Fast Fourier Transform second harmonic generation microscopy as a tool to determine the directionality of corneal stroma as a function of depth. Our results also display the position dependent difference of corneal stroma architecture. ? COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.
Subjects
Collagen; Fast Fourier transforms; Harmonic analysis; Microscopic examination; Musculoskeletal system; Network layers; Nonlinear optics; X ray scattering; Bulk structure; Connective tissues; Cornea; Depth dependents; Fibrillar collagens; Layered network; Position dependents; Second harmonic generation microscopies (SHG); Harmonic generation
Type
conference paper