Analysis of experience-regulated transcriptome and imprintome during critical periods of mouse visual system development reveals spatiotemporal dynamics
Journal
Human Molecular Genetics
Journal Volume
27
Journal Issue
6
Pages
1039-1054
ISSN
0964-6906
Date Issued
2018-05-15
Author(s)
Hsu C.-L.
Chou C.-H.
Huang S.-C.
Lin C.-Y.
Lin M.-Y.
Tung C.-C.
Lin C.-Y.
Lai I.P.
Zou Y.-F.
Youngson N.A.
Lin S.-P.
Abstract
Visual system development is light-experience dependent, which strongly implicates epigenetic mechanisms in light-regulated maturation. Among many epigenetic processes, genomic imprinting is an epigenetic mechanism through which monoallelic gene expression occurs in a parent-of-origin-specific manner. It is unknown if genomic imprinting contributes to visual system development. We profiled the transcriptome and imprintome during critical periods of mouse visual system development under normal- and dark-rearing conditions using B6/CAST F1 hybrid mice. We identified experience-regulated, isoform-specific and brain-region-specific imprinted genes. We also found imprinted microRNAs were predominantly clustered into the Dlk1-Dio3 imprinted locus with light experience affecting some imprinted miRNA expression. Our findings provide the first comprehensive analysis of light-experience regulation of the transcriptome and imprintome during critical periods of visual system development. Our results may contribute to therapeutic strategies for visual impairments and circadian rhythm disorders resulting from a dysfunctional imprintome.
SDGs
Publisher
Oxford University Press
Type
journal article