行政院國家科學委員會補助專題研究計畫成果報告:台灣地區犬脫髓鞘性腦炎之研究
Date Issued
2001
Date
2001
Author(s)
DOI
892313B002196
Abstract
Canine distemper (CD) is a highly contagious viral disease, which has been reported in
dogs for many years in Taiwan. Ever since 1995, apart from systemic CD, there has been
continuous emergence of CD-like demyelinating encephalitis in adult dogs. The goal of this
study is to identify CD in Taiwan using pathology and RT-PCR as diagnostic tools and to
assay 2´, 3´-cyclic nucleotide 3´-phosphohydrolase (CNP) activity of cerebrospinal fluid and
brain tissue with canine distemper virus infection. This report collected 33 cases from animal
hospitals throughout the country as well as from the dog pounds. The affected dogs died either
natural cause or by euthanasia. All cases were conducted to perform pathological investigation,
ABC immunohistochemistry, luxol-fast blue cresyl echt violet (LFB-CEV) histochemistry as
well as transmissible electron microscopy. Phosphoprotein gene of canine distemper virus
(CDV) was used as the target gene for RT-PCR. Under histopathological examination, 16
cases (48.48%) were diagnosed as CD. With the aid of RT-PCR, 27 cases (81.82%) were
diagnosed as CDV positive. This study also found that 2 cases of demyelinating encephalitis
were diagnosed as CDV infection with histopathology, LEB-CEV stain and RT-PCR. On
conclusion, both systemic and demyelinating CD were identified in Taiwan using RT-PCR
along with histopathology. Combination of the two techniques not only raises CD diagnostic
rate, but also provides a more rapid, specific, and sensitive method to confirm CDV infection.
Additionally, spectrophotography was employed in the CNP assay of the brain tissues and
cerebrospinal fluid. The results showed that CNP activity in CD infected dogs with
demyelinating lesion had a significant elevation in the CSF (p<0.05). On the other hand, CD
infected dogs with or without demyelinating lesion demonstrated a significant decline in CNP
activity (p<0.05). Based on the results, CNP assay will be potentially to reflect status of
myelin loss and diagnose demyelinating diseases.
Subjects
canine distemper
demyelinating encephalitis
RT-PCR
CNP assay
Publisher
臺北市：國立臺灣大學獸醫學系暨研究所
Type
report
File(s)![Thumbnail Image]()
Loading...
Name
892313B002196.pdf
Size
58.3 KB
Format
Adobe PDF
Checksum
(MD5):91fa092a257cb180f978436b246348ff