Capping 1,3-propanedithiol to boost the antibacterial activity of protein-templated copper nanoclusters
Journal
Journal of Hazardous Materials
Journal Volume
389
Date Issued
2020
Author(s)
Abstract
We have prepared copper nanoclusters (Cu NCs) in the presence of bovine serum albumin (BSA) and 1,3-propanedithiol (PDT). The PDT/BSA–Cu NCs possess great activities against different types of bacteria, including non-multidrug-resistant bacteria (Escherichia coli, Salmonella Enteritidis, Pseudomonas aeruginosa, and Staphylococcus aureus) and multidrug-resistant bacteria (methicillin-resistant S. aureus). Their minimal inhibitory concentration (MIC) values are at least 242-fold and 10-fold lower than that of the free PDT and BSA–Cu NCs, respectively. The PDT/BSA–Cu NCs are strongly bound to the bacterial membrane, in which they induce the generation of ascorbyl (Asc[rad]) and perhydroxyl (HOO[rad]) radicals that result in disruption of their membrane integrity. At a concentration of 100-fold higher than their MIC for Escherichia coli, the PDT/BSA–Cu NCs exhibit negligible cytotoxicity towards the tested mammalian cells and show insignificant hemolysis. We have further demonstrated that low-cost PDT/BSA–Cu NCs-coated carbon fiber fabrics (CFFs) are effective against antibacterial growth, showing their great potential for antifouling applications. ? 2019 Elsevier B.V.
Subjects
Carbon fibers; Escherichia coli; Free radicals; Mammals; Microwave integrated circuits; Nanoclusters; Proteins; Salmonella; Textiles; Anti-bacterial activity; Antibacterial textiles; Bovine serum albumins; Copper nanoclusters; Minimal inhibitory concentration; Protein templates; Pseudomonas aeruginosa; Salmonella enteritidis; Copper compounds; 1,3 propanedithiol; antibiotic agent; bovine serum albumin; carbon fiber; copper; thiol derivative; 1,3-propanedithiol; antiinfective agent; copper; nanomaterial; propane; thiol derivative; antimicrobial activity; bacterium; chemical compound; cluster analysis; copper; free radical; ligand; nanoparticle; protein; antibacterial activity; bacterial growth; bacterial membrane; controlled study; Escherichia coli; matrix-assisted laser desorption-ionization mass spectrometry; minimum inhibitory concentration; nonhuman; photoluminescence; Pseudomonas aeruginosa; Salmonella enterica serovar Enteritidis; Staphylococcus aureus; chemistry; drug effect; Gram negative bacterium; Gram positive bacterium; microbial sensitivity test; microbial viability; microbiology; Bacteria (microorganisms); Bovinae; Escherichia coli; Mammalia; Pseudomonas aeruginosa; Salmonella enteritidis; Staphylococcus aureus; Anti-Bacterial Agents; Carbon Fiber; Copper; Gram-Negative Bacteria; Gram-Positive Bacteria; Microbial Sensitivity Tests; Microbial Viability; Nanostructures; Propane; Serum Albumin, Bovine; Sulfhydryl Compounds
Type
journal article