Fecal pollution mediates the dominance of stochastic assembly of antibiotic resistome in an urban lagoon (Yundang lagoon), China
Journal
Journal of Hazardous Materials
Journal Volume
417
Date Issued
2021
Author(s)
Abstract
Sewage and fecal pollution cause antibiotic resistance genes (ARGs) pollution in urban lagoons. Seasonality also affects ARG dynamics. However, knowledge of factors controlling ARG community assembly across seasons is still limited. Here, we revealed the seasonal variation of ARGs and depict the underlying assembly processes and drivers via high-throughput quantitative PCR in an urban lagoon, China. A higher richness and abundance of ARGs were observed in summer and winter compared to spring and fall (Kruskal-Wallis test, P < 0.05). Both ARG and prokaryotic communities were mainly governed by stochastic processes, however, these processes drove ARGs and prokaryotes differently across seasons. Stochastic processes played a more dominant role in shaping ARG communities in summer (average stochasticity: 83%) and winter (75%), resulting in a stable antibiotic resistome. However, no such seasonal pattern of stochastic processes was determined for prokaryotes, indicating a decoupling of the assembly process of ARGs and prokaryotes. Moreover, fecal microorganisms (e.g., Bacteroidetes and Faecalibacterium) mediated the stochastic processes of ARG profiles, via enhancement of prokaryotic biomass and mobile genetic element abundances. The tnpA-07 transposase was the key for the horizontal gene transfer. These findings will enhance our understanding of how fecal pollution shapes ARG community assembly in urban lagoons. ? 2021 Elsevier B.V.
Subjects
antibiotic resistance; community structure; dominance; feces; lagoon; polymerase chain reaction; prokaryote; seasonal variation; species richness; stochasticity; China; Bacteroidetes; Faecalibacterium; Prokaryota
Other Subjects
Antibiotics; Genes; Lakes; Microorganisms; Polymerase chain reaction; Random processes; Sewage; Antibiotic resistance genes; Assembly process; Community assembly; Fecal pollution; Prokaryotic community; Prokaryotics; Stochastic assembly; Stochastics; Urban lagoon; Yundang lagoons; Stochastic systems; aminoglycoside; ammonia; antibiotic agent; arginine; bacterial protein; beta lactamase; cellulose; chloramphenicol; cml a1 protein; dissolved oxygen; DNA topoisomerase (ATP hydrolysing) B; ermb protein; humm2 protein; integrase; macrolide lincosamie streptogamin b; mtcytb protein; nitrite; nitrogen; phosphorus; ribosome RNA; RNA 16S; sulfonamide; surface water; tete protein; tetracycline; tnp a 07; transposase; unclassified drug; vancomycin; antiinfective agent; antibiotic resistance; community structure; dominance; feces; lagoon; polymerase chain reaction; prokaryote; seasonal variation; species richness; stochasticity; Actinobacteria; Alphaproteobacteria; antibiotic resistance; antibiotic resistome; Article; Bacteroides; Bacteroidetes; biomass; China; Clostridium; comparative study; controlled study; cyanobacterium; Eubacterium; Faecalibacterium; fecal pollution; feces microflora; Firmicutes; Gammaproteobacteria; horizontal gene transfer; lagoon; mobile genetic element; nonhuman; Parabacteroides; Prevotella; prokaryote; real time polymerase chain reaction; Ruminococcus; seasonal variation; sewage; spring; stochastic model; summer; water pollution; winter; antibiotic resistance; bacterial gene; genetics; pollution; China; Bacteroidetes; Faecalibacterium; Prokaryota; Anti-Bacterial Agents; China; Drug Resistance, Microbial; Environmental Pollution; Genes, Bacterial
Type
journal article