Cell Adhesion and Proliferation on Binary Self-Assembled Monolayers Modified Glass
Date Issued
2014
Date
2014
Author(s)
Kuo, Yu-Ting
Abstract
With the development of medicine, cell behaviors on artificial surface have gained attention among researchers. Cell adhesion is central to many cell behaviors including motility, survival and differentiation. Surface properties of artificial surface are known to be one of the key factors in cell adhesion. Compared with other artificial surface usually used in cell research such as polystyrene, PMMA, and fiber, self-assembled monolayers are known for its excellent ability to modify surface.
To acquire better control of surface properties, mixed functional groups of silanes are introduced to glass surface. In this study, different ratio of 3-aminopropyltrimethoxysilane and 3-mercaptopropyltrimethoxysilane are applied to glass substrate, surfaces with a series of zeta potentials from +24 to -155 mV at pH 7.4 are obtained.
The surface chemical composition of binary SAMs is quantified using x-ray photoelectron spectroscopy, while that of zeta potential can be found in reference. NIH3T3 cells are chosen to culture on these binary-SAM modified glass and incubated for several different time periods. The population density, proliferation rate and shape of cells adhered on surfaces of various potentials are examined using optical microscope, fluorescence microscope and scanning electron microscope. The results showed that surface with higher positive potential promotes cell adhesion and proliferation. On the contrary, more negative the zeta potential is, worse the cells adhere and grow. And zeta potential have little effect on proliferation rate.
To acquire better control of surface properties, mixed functional groups of silanes are introduced to glass surface. In this study, different ratio of 3-aminopropyltrimethoxysilane and 3-mercaptopropyltrimethoxysilane are applied to glass substrate, surfaces with a series of zeta potentials from +24 to -155 mV at pH 7.4 are obtained.
The surface chemical composition of binary SAMs is quantified using x-ray photoelectron spectroscopy, while that of zeta potential can be found in reference. NIH3T3 cells are chosen to culture on these binary-SAM modified glass and incubated for several different time periods. The population density, proliferation rate and shape of cells adhered on surfaces of various potentials are examined using optical microscope, fluorescence microscope and scanning electron microscope. The results showed that surface with higher positive potential promotes cell adhesion and proliferation. On the contrary, more negative the zeta potential is, worse the cells adhere and grow. And zeta potential have little effect on proliferation rate.
Subjects
細胞貼附
細胞生長
自組裝單層膜
混合官能基
表面電位
Type
thesis
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