Effects of Stress on the Suspension Cell Culture of inseng and Embryogenesis Culture, Regeneration of the Celery
Date Issued
2007
Date
2007
Author(s)
Chan, Hsiao-Sung
DOI
zh-TW
Abstract
Investigation on the optimum conditions for suspension culture of undifferentiated plant cells producing secondary metabolites (Panax ginseng producing ginseng saponin) as well as for inducing differentiated somatic embryogenic cells (Apium graveolens var. dulce, Tall Utah) were conducted.
Shear stress effects on Panax ginseng suspension culture were performed using 250 mL shake flask and 7 L stirred tank reactor (STR). Biomass concentration and saponin productivity were determined at various operation conditions. The biomass concentration attained in shake flask was 12 g DW/L which was higher than that in STR (9.65, 8.83 and 8.47 g DW/L for agitation rate 200, 300 and 400 rpm, respectively). However, the productivity of total saponin in STR at 300 rpm (2.34 % DW) was much higher than that in shake flask (0.89 % DW). Taking power consumptions in the shake flask and STR as an index of shear stress intensity, its effect on the performance of cell culture in different bioreactor systems were compared. For shake flask, an empirical equation proposed by Büchs et al., (2000) was used to estimate its power consumption. As for STR, a strain gauge type torque meter was employed to estimate the power consumption. Furthermore, a solid-liquid (
Subjects
人蔘懸浮細胞
西洋芹菜
體胚發生
逆境
Ginseng Suspension Cell
Celery
Embryogenesis
Stress
Type
thesis