Effects of Glutamine Supplementation on Innate Immune Response in Rats with Gut-Derived Sepsis
Resource
BRITISH JOURNAL OF NUTRITION v.91 n.3 pp.423-429
Journal
BRITISH JOURNAL OF NUTRITION
Journal Volume
v.91
Journal Issue
n.3
Pages
423-429
Date Issued
2004
Date
2004
Author(s)
YEH, SUNG-LING
LAI, YU-NI
SHANG, HUEY- FANG
LIN, MING-TSAN
Abstract
The present study examined the effect of glutamine (Gln)- enriched diets before sepsis or Gln-containing total parenteral nutrition (TPN) after sepsis, or both, on the phagocytic activity and blood lymphocyte subpopulation in rats with gut-derived sepsis. Rats were assigned to a control group or one of four experimental groups. The control group and groups 1 and 2 were fed a semipurified diet; groups 3 and 4 had part of casein replaced by Gin. After feeding the diets for 10 d, sepsis was induced by caecal ligation and puncture (CLP); TPN was maintained for 3 d after CLP. The control group and groups 1 and 3 were infused with conventional TPN and groups 2 and 4 were supplemented with Gin in the TPN solution. All rats were killed 3 d after CLP or sham operation to examine their immune responses. The results showed that compared with the control group, the phagocytic activities of peritoneal macrophages were enhanced in groups 3 and 4, but not in groups 1 and 2. The proportion of CD3(+) cells in group 1 was significantly lower (P<0.05) than that of the control group, whereas no differences were observed among the control and Gln- supplemented groups. The CD4(+) cell proportion was significantly lower (P <0.05) in group I compared with the control group and groups 3 and 4. These findings suggest that Gln-enriched diets before CLP significantly enhanced peritoneal macrophage phagocytic activity, preserved CD4(+) cells and maintained blood total T lymphocytes in gut-derived sepsis. However, parenteral Gin administration after caecal ligation and puncture had no favourable effects on modulating immune response in septic rats.
Subjects
glutamine
gut-derived sepsis
phagocytosis
lymphocyte subpopulation
Type
journal article