A rapid and portable sensor based on protein-modified gold nanoparticle probes and lateral flow assay for naked eye detection of mercury ion
Resource
Microelectron. Eng., 97, 294-296
Journal
Microelectronic Engineering
Journal Volume
97
Pages
294-296
Date Issued
2012
Author(s)
Wu, Chung-Shu
Huang, Chung-Chih
Liang, Jie-Chian
Wang, Hsiao-Ting
Tang, Pin-Ting
Lin, Lih-Yuan
Ko, Fu-Hsiang
Abstract
Contamination of the environment with heavy metal ions has been an important worldwide concern for decades. Among them, mercury ion (Hg 2+) is one of the heavy metal ion maintained in the detection because of its toxicity. In this study, we have designed the gold nanoparticle (AuNP) probes of carboxylic modified protein and employed the lateral flow assay to detect the Hg 2+ without complicated instrumentations and technical expertise. The captured AuNP probes on the test zone and control zone of the sensor produced the characteristic red bands, enabling visual discrimination of Hg 2+ concentrations. The demand of Hg 2+ analysis in environmental applications requires parallel analytical strategies; such a method with simplicity and rapidity has enormous potential for the application of Hg 2+ monitoring in environment, water, and food samples. © 2012 Elsevier B.V. All rights reserved.
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