Development of Electrical Methodology to Study DNA Characterization in Microfluidic on PMMA Chips
Date Issued
2004
Date
2004
Author(s)
Mao, Cheng-Hsin
DOI
zh-TW
Abstract
Abstract
This study is bases on capillary electrophoresis method and incorporated with the technique of MEMS to design and fabricate several kind different dimensions micro-fluidic for polymer material; We design and fabricate a electrical measurement system by ourselves. We loaded DNA in the micro-fluidic on the PMMA for capillary electrophoresis and did electrical measurement, for realization the electrical characterization about DNA.
The capillary electrophoresis method for DNA is processing in the cross type micro-fluidic on the PMMA. The cross type micro-fluidic is using the technique of MEMS to fabricate PMMA chip, about 150 µm width 35 µm depth and 300 µm width 35 µm depth two different size cross micro-fluidic. We loaded a small amount of DNA solution into the micro-fluidic. We applied voltage in the micro-fluidic from end to end for DNA do capillary electrophoresis to measurement DNA electrical characterization.
The measurement plate was designed and fabricated by ourselves for the electrical characterization measurement. It’s can through four test terminal connect to 4D controllable and movable test tungsten needle, to contact micro-fluidic on the PMMA chip in the plate central. We loaded into DD water and DNA aqueous solution into micro-fluidic for measurement I-V and I-T electrical characterization. Because of the DNA at same mer under different supply voltage and the DNA at different mer under same supply voltage will be different result. According to these results to discover and prove the distinct divergences in different DNA molecular.
Subjects
晶片
電性量測法
微流道
PMMA
Microfluidics
DNA
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-93-P91921009-1.pdf
Size
23.31 KB
Format
Adobe PDF
Checksum
(MD5):baeffdaa5483f47e39f3ab8c9f43c035