Study of the relationship between Lcn2 protein-activated macrophage and endometriosis
Date Issued
2009
Date
2009
Author(s)
Lin, Che-Yu
Abstract
Lcn2 protein, a lipocalin, is one of the acute phase proteins. According to our previous data, ovarian steroids regulate the expression of Lcn2 during the estrous cycle. Besides, Lcn2 protein has been found to induce IL-8 and MCP-1 expression in human endometrial carcinoma cell line (RL95-2). There are substantial evidences showing that the progression of endometriosis is estrogen-dependent and that increased levels of cytokines such as IL-8 and MCP-1 are associated with endometriosis formation. In this study, a mouse model of surgically induced endometriosis and a mouse macrophage cell line (RAW264.7) were used to investigate the role of Lcn2 during the development of endometriosis. We demonstrated that during endometriosis formation, the levels of Lcn2 were increased in the peritoneal fluid. Treatment of RAW264.7 cells with Lcn2 resulted in the activation of macrophages. This was accompanied by the elevation of iNOS and TNF-α and the secretion of cytokines, including IL-6, GCSF, RANTES, COX-2, MIP-2 and MCP-1, all of which are factors that facilitate the development of endometriosis. In conclusion, Lcn2 may trigger the activation of macrophages in the peritoneal fluid and thus promote the development of endometriosis.
Subjects
Lcn2
macrophage
endometriosis
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