Enhancement of anticancer properties of cruciferous sprouts by supplementing with different sulphur salts
Date Issued
2009
Date
2009
Author(s)
Lin, Jia-Ching
Abstract
Edible sprouts are one of the potentially new functional foods. Especially, cruciferous sprouts contain high concentration of glucosinolates, which are potent source of protective chemicals against cancer. Glucosinolates can induce the phase II enzyme system thus inhibiting the growth of tumor. Besides, it has been observed that supplementing the nutrient medium (water/soil) of sprouting seeds can be of great benefit for improving the nutritional quality of the sprouts. The purpose of our efforts was to enhance the antioxidant and anticancer properties of cruciferous sprouts by supplementing the growth medium with different sulphur salts (ammonium sulfate, magnesium sulfate and sodium thiosulfate), which could increase the main concentration of the bioactive compounds – glucosinolates. Three cruciferous sprouts used in our studies namely, cabbage, broccoli and radish, showed an increase in glucosinolates content during sprouting with a maximum at day 12. Sulfur salt concentrations were optimized and finally used at 20, 40 and 60 kg/ha, where the sprout yield was not adversely affected by salt concentrations. In case of radish sprouts supplemented with sodium thiosulfate (60 kg/ha), the aqueous extracts showed higher glucosinolate content (1.996 mM) than ethanolic extract (0.699 mM). Also there was a 5.58 fold increase as compared to control. Further it showed very good DPPH and ABTS+． scavenging activity, i.e., 89% and 96% respectively. The anticancer activity of radish aqueous extract was also check and was found to suppress the growth of Hep G2 hepatoma cells by 53% and CT-26 colon cancer cells by 78%. Similar increase of glucosinolate content and free radical scavenging activity were obtained in case of cabbage and broccoli sprouts. Accordingly, the cancer cell inhibition for cabbage sprouts was found to be 72% and 84% for Hep G2 and CT-26 cells, respectively. While for broccoli sprouts the cancer cell inhibition was found to be 67% and 77% for Hep G2 and CT-26 cells, respectively. Thus the supplementation of different cruciferous sprouts with sulfur salts was found to be very effective in increasing their anticancer properties against different cancer cell lines.
Subjects
sprout
glucosinolates
cabbage
broccoli
radish
sulphur salt
SDGs
Type
thesis
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